Faculty of Science, Sichuan Agricultural University, Yaan 625014, PR China.
Anal Biochem. 2010 Mar 1;398(1):120-2. doi: 10.1016/j.ab.2009.11.006. Epub 2009 Nov 10.
A multifunctional magnetic nanoparticle (MNP)-assisted bioseparation method was developed to isolate plasmid DNA (pDNA) from Escherichia coli culture. Using the pH-sensitive carboxyl-modified magnetic nanoparticles, both cell capture and the subsequent removal of genomic DNA/protein complex after lysis can be achieved simply by magnetic separation. Furthermore, the yield and purity of pDNA extracted by MNPs are comparable to those obtained using organic solvents or commercial kits. This time- and cost-effective protocol does not require centrifugation or precipitation steps and has the potential for automated DNA extraction, especially within miniaturized lab chip applications.
一种多功能磁性纳米颗粒（MNP）辅助的生物分离方法被开发出来，用于从大肠杆菌培养物中分离质粒 DNA（pDNA）。利用 pH 敏感的羧基修饰的磁性纳米颗粒，可以通过简单的磁分离实现细胞捕获和随后裂解后基因组 DNA/蛋白质复合物的去除。此外，MNP 提取的 pDNA 的产量和纯度与使用有机溶剂或商业试剂盒获得的相当。该方法省时、经济，不需要离心或沉淀步骤，并且具有自动化 DNA 提取的潜力，尤其适用于微型化实验室芯片应用。
