Herbal Medicine EBM Research Center, Korea Institute of Oriental Medicine, Daejeon, Republic of Korea.
Regul Toxicol Pharmacol. 2011 Apr;59(3):391-6. doi: 10.1016/j.yrtph.2011.01.002. Epub 2011 Jan 15.
Yukmijihwang-tang (Liu wei di huang tang, Rokumigan; YMJ) has been used for body enrichment; however, little toxicological evaluation of YMJ has been performed to assure its safety for clinical treatment. To increase the safety information for YMJ, its genotoxicity was evaluated. There was no increase in the number of revertant colonies in four strains of Salmonella typhimurium or one strain of Escherichia coli at any concentration of YMJ studied, regardless of the including when dosed with YMJ metabolized with and S-9 microsomal fraction. YMJ significantly increased structural aberrations in Chinese hamster lung (CHL) cells at the high concentrations (2500 and 5000 μg/ml) in the presence or absence of metabolic activation by the S-9 microsomal fraction. Oral administration of YMJ at doses up to 2000 mg/kg did not increase the incidence of micronucleated polychromatic erythrocytes in bone marrow. These results suggest that YMJ is not genotoxic at the proper dose.
育阴蠲浊汤（六味地黄汤、六君子汤；YMJ）被用于补益身体；然而，为了保证其在临床治疗中的安全性，对 YMJ 的毒理学评价很少。为了增加 YMJ 的安全性信息，对其遗传毒性进行了评估。无论是否用 S-9 微粒体部分代谢，YMJ 在任何研究浓度下，对 4 株鼠伤寒沙门氏菌或 1 株大肠杆菌的回复菌落数均无增加。在存在或不存在 S-9 微粒体部分代谢激活的情况下，YMJ 在高浓度（2500 和 5000μg/ml）时显著增加中国仓鼠肺（CHL）细胞的结构异常。YMJ 口服剂量高达 2000mg/kg 时，不会增加骨髓中多色红细胞微核的发生率。这些结果表明，YMJ 在适当剂量下没有遗传毒性。
