Suntory Business Expert Limited, Shimamoto-cho, Mishima-gun, Osaka 618-8503, Japan.
Mutat Res. 2011 Feb 3;719(1-2):21-8. doi: 10.1016/j.mrgentox.2010.10.001. Epub 2010 Oct 16.
Sesamin is a major lignan that is present in sesame seeds and oil. Sesamin is partially converted to its stereoisomer, episesamin, during the refining process of non-roasted sesame seed oil. We evaluated the genotoxicity of these substances through the following tests: a bacterial reverse mutation assay (Ames test), a chromosomal aberration test in cultured Chinese hamster lung cells (CHL/IU), a bone marrow micronucleus (MN) test in Crlj:CD1 (ICR) mice, and a comet assay using the liver of Sprague-Dawley (SD) rats. Episesamin showed negative results in the Ames test with and without S9 mix, in the in vitro chromosomal aberration test with and without S9 mix, and in the in vivo comet assay. Sesamin showed negative results in the Ames test with and without S9 mix. In the in vitro chromosomal aberration test, sesamin did not induce chromosomal aberrations in the absence of S9 mix, but induced structural abnormalities at cytotoxic concentrations in the presence of S9 mix. Oral administration of sesamin at doses up to 2.0g/kg did not cause a significant increase in either the percentage of micronucleated polychromatic erythrocytes in the in vivo bone marrow MN test or in the % DNA in the comet tails in the in vivo comet assay of liver cells. These findings indicate that sesamin does not damage DNA in vivo and that sesamin and episesamin have no genotoxic activity.
芝麻素是一种存在于芝麻种子和油中的主要木脂素。芝麻素在未烘焙芝麻油的精炼过程中会部分转化为其对映异构体表芝麻素。我们通过以下测试评估这些物质的遗传毒性：细菌回复突变试验（Ames 试验）、培养的中国仓鼠肺细胞（CHL/IU）染色体畸变试验、Crlj：CD1（ICR）小鼠骨髓微核（MN）试验和使用 Sprague-Dawley（SD）大鼠肝脏的彗星试验。表芝麻素在有或没有 S9 混合物的 Ames 试验中、在有或没有 S9 混合物的体外染色体畸变试验中和在体内彗星试验中均呈阴性结果。芝麻素在有或没有 S9 混合物的 Ames 试验中均呈阴性结果。在体外染色体畸变试验中，芝麻素在没有 S9 混合物的情况下没有诱导染色体畸变，但在存在 S9 混合物的情况下，在细胞毒性浓度下诱导结构异常。芝麻素口服剂量高达 2.0g/kg 时，在体内骨髓 MN 试验中多色性红细胞微核的百分比或体内肝细胞彗星试验中彗星尾部的%DNA 均无显著增加。这些发现表明，芝麻素不会在体内损伤 DNA，芝麻素和表芝麻素没有遗传毒性活性。
