Saïle R, Kabbaj O, Visvikis S, Steinmetz J, Steinmetz A, Férard G, Fruchart J C, Métais P
INSERM U.325, Institut Pasteur de Lille, France.
J Clin Chem Clin Biochem. 1990 Aug;28(8):519-25. doi: 10.1515/cclm.1990.28.8.519.
In five severely head-injured patients we determined the plasma concentrations of apolipoproteins serum amyloid A, A-I, A-II, C-III, and B, prealbumin and C-reactive protein on day 1, 5, 10 and 15 after head injury where possible. A dramatic increase in apolipoprotein serum amyloid A up to a mean plasma level of 0.764 g/l was accompanied by a considerable decrease in apolipoprotein A-I, apolipoprotein A-II and apolipoprotein C-III concentrations. The variations observed by immunological methods were confirmed by two-dimensional gel electrophoresis performed on plasma and different lipoprotein fractions. In addition to its association with high density lipoproteins, apolipoprotein serum amyloid A was also found with lipoproteins of low and very low density. Two-dimensional electrophoresis also showed the presence of several different serum amyloid A-peptides not seen in plasmas from healthy subjects. We propose that apolipoprotein serum amyloid A may be responsible for the decrease of the main HDL apolipoproteins in head-injured patients.
在五名重度颅脑损伤患者中，我们尽可能在伤后第1天、第5天、第10天和第15天测定了血浆中载脂蛋白血清淀粉样蛋白A、A-I、A-II、C-III和B、前白蛋白以及C反应蛋白的浓度。载脂蛋白血清淀粉样蛋白A显著增加，平均血浆水平高达0.764 g/l，同时载脂蛋白A-I、载脂蛋白A-II和载脂蛋白C-III浓度大幅下降。通过免疫方法观察到的变化经对血浆和不同脂蛋白组分进行二维凝胶电泳得到证实。除了与高密度脂蛋白相关外，载脂蛋白血清淀粉样蛋白A还存在于低密度和极低密度脂蛋白中。二维电泳还显示出几种在健康受试者血浆中未见的不同血清淀粉样蛋白A肽段。我们认为，载脂蛋白血清淀粉样蛋白A可能是颅脑损伤患者主要高密度脂蛋白载脂蛋白减少的原因。
