Metal ion (Ag(+), Cd(2+), Zn(2+)) modified CdS quantum dots (QDs) were synthesized and used for Cu(2+) sensing. Modification by these metal ions could enhance the PL intensity of CdS QDs with the extent of the PL enhancement being related to the concentration of the metal ions. Different metal ion (Ag(+), Cd(2+), Zn(2+)) modified CdS QDs also showed different analytical characteristics for Cu(2+) sensing. In particular, Ag( + ) modified CdS QDs showed greatly enhanced sensitivity for Cu(2+) determination than did the unmodified CdS QDs. A limit of detection (LOD) of 2.0 × 10(-10) M was obtained for Ag(+) modified CdS QDs, which is the lowest LOD obtained using QDs as fluorescence probes for Cu(2+) sensing. This study demonstrates the important role of surface state of QDs in fluorescence sensing.