Kawate Noritoshi, Watanabe Kensuke, Uenaka Kazuhiro, Takahashi Masahiro, Inaba Toshio, Tamada Hiromichi
Department of Advanced Pathobiology, Osaka Prefecture University, Osaka, Japan.
J Reprod Dev. 2011 Apr;57(2):267-72. doi: 10.1262/jrd.10-066t. Epub 2011 Jan 14.
The objectives of this study were 1) to determine the effects of adding a CIDR to the Ovsynch protocol on plasma concentrations of estradiol-17β and progesterone and conception in dairy cows with cystic ovarian diseases and 2) to examine associations among the estradiol-17β and progesterone concentrations and conception. Cows were diagnosed as having cystic ovarian diseases if they were found to have a cystic follicle (diameter ≥25 mm) without a corpus luteum by two palpations per rectum with an interval for 7 to 14 days. They were treated with either the Ovsynch (GnRH on Day 0, PGF(2α) on Day 7 and GnRH on Day 9, with AI on Day 10; n=15) or Ovsynch+CIDR protocol (Ovsynch protocol plus a CIDR from Day 0 to Day 7; n=23). Plasma estradiol-17β concentrations were determined on Days 0, 7 and 9, and plasma progesterone concentrations were determined on Days 0, 7, 9 and 17. The plasma estradiol-17β and progesterone concentrations at all of the days examined and conception rates did not differ significantly between the two timed AI protocols. The progesterone concentrations on Day 17 and conception rates were lower (P<0.05) for cows with low concentrations of estradiol-17β (<2 pg/ml) on Day 9 than for cows with high concentrations of estradiol-17β (≥2 pg/ml). The present study suggests that, in dairy cows with cystic ovarian diseases, addition of a CIDR to the Ovsynch protocol had no remarkable effects on plasma estradiol-17β and progesterone concentrations during and after the treatments or on conception after timed AI. This study indicates that the low plasma estradiol-17β concentration at the second administration of GnRH in the protocols can be a predictor for impaired luteal formation and lower likelihood of pregnancy in dairy cows with cystic ovarian diseases.
1）确定在患有卵巢囊肿疾病的奶牛中，在Ovsynch方案中添加孕酮释放阴道环（CIDR）对血浆17β-雌二醇和孕酮浓度以及受孕的影响；2）研究17β-雌二醇和孕酮浓度与受孕之间的关联。如果通过直肠触诊发现奶牛有一个直径≥25mm且无黄体的囊性卵泡，且在7至14天的间隔内进行两次直肠触诊，则诊断该奶牛患有卵巢囊肿疾病。它们分别接受Ovsynch方案（第0天注射促性腺激素释放激素（GnRH），第7天注射前列腺素F2α（PGF(2α)），第9天注射GnRH，第10天进行人工授精（AI）；n = 15）或Ovsynch+CIDR方案（Ovsynch方案加上从第0天到第7天放置一个CIDR；n = 23）治疗。在第0、7和9天测定血浆17β-雌二醇浓度，在第0、7、9和17天测定血浆孕酮浓度。在两种定时人工授精方案之间，所有检测日的血浆17β-雌二醇和孕酮浓度以及受孕率均无显著差异。第9天血浆17β-雌二醇浓度低（<2 pg/ml）的奶牛，其第17天的孕酮浓度和受孕率低于血浆17β-雌二醇浓度高（≥2 pg/ml）的奶牛（P<0.05）。本研究表明，在患有卵巢囊肿疾病的奶牛中，在Ovsynch方案中添加CIDR对治疗期间和治疗后的血浆17β-雌二醇和孕酮浓度或定时人工授精后的受孕没有显著影响。本研究表明，在这些方案中第二次注射GnRH时血浆17β-雌二醇浓度低可能是患有卵巢囊肿疾病的奶牛黄体形成受损和怀孕可能性较低的一个预测指标。
