两光子荧光溶酶体生物成像技术及胶束包裹的荧光探针应用
Two-photon fluorescence lysosomal bioimaging with a micelle-encapsulated fluorescent probe.
机构信息
Department of Chemistry, University of Central Florida, 4000 Central Florida Boulevard, Orlando, FL 32816, USA.
出版信息
J Fluoresc. 2011 May;21(3):1223-30. doi: 10.1007/s10895-010-0801-3. Epub 2011 Jan 18.
We report two-photon fluorescence microscopy (2PFM) imaging and in vitro cell viability of a new, efficient, lysosome-selective system based on a two-photon absorbing (2PA) fluorescent probe (I) encapsulated in Pluronic® F-127 micelles. Preparation of dye I was accomplished via microwave-assisted synthesis, resulting in improved yields and reduced reaction times. Photophysical characterization revealed notable 2PA efficiency of this probe.
我们报告了基于两亲性嵌段共聚物 Pluronic® F-127 胶束包封的新型高效、溶酶体选择性的两光子吸收（2PA）荧光探针（I）的双光子荧光显微镜（2PFM）成像和体外细胞活力。通过微波辅助合成制备了染料 I，从而提高了产率并缩短了反应时间。光物理特性研究表明该探针具有显著的 2PA 效率。
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