Takahashi Y, Miura T
Basic Medical Sciences Division, National Institute for Environmental Studies, Ibaraki, Japan.
Toxicol Lett. 1990 Dec;54(2-3):327-35. doi: 10.1016/0378-4274(90)90200-6.
The effects of 2 weeks exposures of rats to 0.2 ppm O3 on pulmonary cytochrome P-450 isozymes were investigated. Two main types of cytochrome P-450 isozymes (cytochromes P-450FI and P-450FII) were separated from lung microsomes of control rats on an anion exchange cellulose column. Antibody raised against cytochrome P-450b, which is the main isozyme of liver obtained from phenobarbital-treated rats, cross-reacted with pulmonary cytochrome P-450FII, but not with cytochrome P-450FI. Ozone exposures caused increases in the content of both cytochromes P-450FI and P-450FII to the same extent two times greater than those of control rats without changes in immunological properties of these isozymes. No pulmonary cytochrome P-450 isozyme other than cytochromes P-450FI and P-450FII was observed in eluate through anion exchange column chromatography. These results indicate that increases in the content of pulmonary cytochrome P-450 of rats exposed to O3 can be ascribed to increase in constitutive types of the isozymes, but not to induction of other types of isozyme.
研究了大鼠暴露于0.2 ppm臭氧2周对肺细胞色素P - 450同工酶的影响。在阴离子交换纤维素柱上，从对照大鼠的肺微粒体中分离出两种主要类型的细胞色素P - 450同工酶（细胞色素P - 450FI和P - 450FII）。针对从苯巴比妥处理的大鼠肝脏中获得的主要同工酶细胞色素P - 450b产生的抗体，与肺细胞色素P - 450FII发生交叉反应，但不与细胞色素P - 450FI发生交叉反应。臭氧暴露使细胞色素P - 450FI和P - 450FII的含量增加到相同程度，比对照大鼠增加了两倍，且这些同工酶的免疫特性没有变化。通过阴离子交换柱色谱法在洗脱液中未观察到除细胞色素P - 450FI和P - 450FII之外的其他肺细胞色素P - 450同工酶。这些结果表明，暴露于臭氧的大鼠肺细胞色素P - 450含量的增加可归因于同工酶组成型类型的增加，而不是其他类型同工酶的诱导。
