Anal Chem. 2011 Feb 15;83(4):1167-72. doi: 10.1021/ac102917f. Epub 2011 Jan 18.
Multiplexed assays that can measure protein biomarkers and internal standards are highly desirable given the potential to reduce false positives and negatives. We report here the use of a chip-based platform that achieves multiplexed immunosensing of the ovarian cancer biomarker CA-125 without the need for covalent labeling or sandwich complexes. The sensor chips allow the straightforward comparison of detectors of different sizes, and we used this feature to scan the microscale size regime for the best sensor size and optimize the limit of detection exhibited down to 0.1 U/mL. The assay has a straightforward design, with readout being performed in a single step involving the introduction of a noncovalently attached redox reporter group. The detection system reported exhibits excellent specificity, with analysis of a specific cancer biomarker, CA-125, performed in human serum and whole blood. The multiplexing of the system allows the analysis of the biomarker to be performed in parallel with an abundant serum protein for internal calibration.
鉴于有可能减少假阳性和假阴性，能够同时测量蛋白质生物标志物和内标物的多重分析是非常理想的。在此，我们报告了一种基于芯片的平台，该平台无需共价标记或三明治复合物即可实现对卵巢癌生物标志物 CA-125 的多重免疫检测。传感器芯片允许对不同大小的探测器进行简单的比较，我们利用这一特点扫描微尺度范围，以找到最佳的传感器尺寸，并将检测下限优化至 0.1 U/mL。该分析具有简单的设计，通过引入非共价结合的氧化还原报告基团来进行单步读出。所报道的检测系统具有出色的特异性，在人血清和全血中分析特定的癌症生物标志物 CA-125。该系统的多重分析允许同时分析生物标志物和用于内部校准的丰富血清蛋白。
