Erdman S H
Department of Pediatrics, Loma Linda University School of Medicine, Calif.
Digestion. 1990;46 Suppl 2:396-402. doi: 10.1159/000200414.
Starvation and difluoromethylornithine (DFMO) administration have profound affects on intestinal proliferation, ornithine decarboxylase activity, and tissue polyamine levels. Diamine oxidase activity may play a role in the regulation of proliferation, and the activity of this enzyme may be influenced by ornithine decarboxylase activity. To determine if diamine oxidase is influenced by starvation and DFM administration, ileal diamine oxidase activities were determined on mucosal homogenates from five groups of rats: fed control, starved for 48 h, fed group receiving DFMO, a starved/refed group, and a starved/refed group receiving DMFO. The homogenates from starved rats were found to have decreased ornithine decarboxylase activity and increased diamine oxidase activity when compared to control values. The homogenates from the DFMO group also were found to have decreased ODC activity however, mucosal diamine oxidase activity was also decreased. Refeeding produced a dramatic increase in ornithine decarboxylase activity and a minimal change in diamine oxidase activity. The preservation of diamine oxidase activity during starvation implies a need for the enzyme not related to mucosal proliferation or digestion. However, in the fed state, diamine oxidase activity may be more dependent on ornithine decarboxylase activity or its reaction product putrescine.
饥饿和给予二氟甲基鸟氨酸（DFMO）对肠道增殖、鸟氨酸脱羧酶活性及组织多胺水平有深远影响。二胺氧化酶活性可能在增殖调节中起作用，且该酶的活性可能受鸟氨酸脱羧酶活性的影响。为确定二胺氧化酶是否受饥饿和DFMO给药的影响，测定了五组大鼠回肠黏膜匀浆中的二胺氧化酶活性：正常饮食对照组、饥饿48小时组、给予DFMO的正常饮食组、饥饿/再喂养组以及给予DFMO的饥饿/再喂养组。与对照值相比，饥饿大鼠的匀浆中鸟氨酸脱羧酶活性降低，二胺氧化酶活性升高。DFMO组的匀浆中ODC活性也降低，然而黏膜二胺氧化酶活性也降低。再喂养使鸟氨酸脱羧酶活性显著增加，而二胺氧化酶活性变化极小。饥饿期间二胺氧化酶活性的维持意味着该酶的需求与黏膜增殖或消化无关。然而，在进食状态下，二胺氧化酶活性可能更依赖于鸟氨酸脱羧酶活性或其反应产物腐胺。
