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采用高效液相色谱-质谱法鉴定和定量分析非洲吊灯树(Balanites aegyptiaca(L)Del)叶和叶瘿中的酚类化合物。

Identification and quantification of phenolic compounds from Balanites aegyptiaca (L) Del (Balanitaceae) galls and leaves by HPLC-MS.

机构信息

Laboratoire de Biochimie et de Chimie Appliquees, UFR/SVT, Universite de Ouagadougou, Ouagadougou, Burkina Faso, Romania.

出版信息

Nat Prod Res. 2011 Jan;25(2):93-9. doi: 10.1080/14786419.2010.482933.

Abstract

Balanites aegyptiaca is a tropical plant which is widely used for medicinal purposes in several African countries, including Burkina Faso. Despite its widespread use, little is known about its phenolic content. This study sought to carry out a screening of the polyphenols from the leaves and galls of B. aegyptiaca. A high-performance liquid chromatography-mass spectrometry method was used to investigate the phenolic content in the parts of the plant studied here. The phenolic acid profile showed the presence of gentisic, p-coumaric, caffeic, ferulic and sinapic acids in the crude and hydrolysed extracts. The flavonoids pattern showed hyperoside, isoquercitrin, rutoside and quercitrin in the crude extract of leaves. Myricetol, quercetol and kaempferol were found after acid hydrolysis of the leaves extract. Ferulic acid, isoquercitrin, rutoside and quercitrin were identified as major phenolic compounds in this study.

摘要

非洲朴是一种热带植物,在包括布基纳法索在内的几个非洲国家被广泛用于药用。尽管它被广泛使用,但人们对其酚类物质的含量知之甚少。本研究旨在对非洲朴的叶和瘿进行多酚筛选。采用高效液相色谱-质谱联用方法对研究植物部分的酚类物质含量进行了研究。酚酸图谱显示,在粗提物和水解提取物中存在龙胆酸、对香豆酸、咖啡酸、阿魏酸和芥子酸。黄酮类图谱显示,在叶的粗提物中存在桃叶珊瑚苷、异槲皮苷、芦丁和槲皮苷。叶提取物经酸水解后发现杨梅醇、槲皮素和山奈酚。本研究鉴定出阿魏酸、异槲皮苷、芦丁和槲皮苷为主要的酚类化合物。

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