West China School of Public Health, Sichuan University, Provincial Key Laboratory for Food Safety Monitoring and Risk Assessment of Sichuan, No. 17, Section 3, South Renmin Road, Chengdu 610041, China.
Food Chem. 2013 Dec 1;141(3):2697-706. doi: 10.1016/j.foodchem.2013.05.009. Epub 2013 May 15.
The study systematically investigated free, conjugate and total phenolics (phenolic acids and flavonoids) in leaves of 19 Chinese and one American sweetpotato cultivars grown in China. Three extraction/hydrolytic methods (direct extraction and acidic and basic hydrolysis) for sample preparation were employed to obtain different forms of phenolics. Twenty-nine phenolics were separated and identified using HPLC-DAD and HPLC-ESI-MS/MS. Three quercetin glycosides were characterised for the first time from this plant. Contents of the principal phenolics identified were determined by the HPLC-DAD procedure, which was validated in terms of linearity, precision, accuracy and limit of detection and quantification. Moreover, to the best of our knowledge, it is the first to reveal and demonstrate artifacts of esterification during acidic methanolic and ethanolic hydrolysis, and chromatographic behaviours, UV spectra and MS data of 20 hydroxycinnamic acid methyl and ethyl esters were obtained using acidic methanolic and ethanolic hydrolysis.
该研究系统地调查了 19 种中国和 1 种美国甘薯品种叶片中的游离、结合和总酚（酚酸和类黄酮）。采用三种提取/水解方法（直接提取以及酸性和碱性水解）进行样品制备，以获得不同形式的酚类化合物。使用 HPLC-DAD 和 HPLC-ESI-MS/MS 分离和鉴定了 29 种酚类化合物。首次从这种植物中鉴定出三种槲皮素糖苷。通过 HPLC-DAD 程序确定了主要酚类化合物的含量，该程序在线性、精密度、准确度和检测限及定量限方面进行了验证。此外，据我们所知，这是首次揭示并证明酸性甲醇和乙醇水解过程中的酯化副产物，以及使用酸性甲醇和乙醇水解获得的 20 种对羟基肉桂酸甲酯和乙酯的色谱行为、紫外光谱和 MS 数据。
