Department of Molecular Cell and Developmental Biology, University of California Los Angeles, Los Angeles, California 90095, USA.
J Comp Neurol. 2011 Mar 1;519(4):661-89. doi: 10.1002/cne.22542.
Most neurons of the central complex belong to 10 secondary (larvally produced) lineages. In the late larva, undifferentiated axon tracts of these lineages form a primordium in which all of the compartments of the central complex can be recognized as discrete entities. Four posterior lineages (DPMm1, DPMpm1, DPMpm2, and CM4) generate the classes of small-field neurons that interconnect the protocerebral bridge, fan-shaped body, noduli, and ellipsoid body. Three lineages located in the anterior brain, DALv2, BAmv1, and DALcl2, form the large-field neurons of the ellipsoid body and fan-shaped body, respectively. These lineages provide an input channel from the optic tubercle and connect the central complex with adjacent anterior brain compartments. Three lineages in the posterior cortex, CM3, CP2, and DPMpl2, connect the posterior brain neuropil with specific layers of the fan-shaped body. Even though all of the compartments of the central complex are prefigured in the late larval brain by the axon tracts of the above-mentioned lineages, the neuropil differentiates during the first 2 days of the pupal period when terminal branches and synapses of secondary neurons are formed. During this phase the initially straight horizontal layers of the central complex bend in the frontal plane, which produces the characteristic shape of the fan-shaped and ellipsoid body. Our analysis provides a comprehensive picture of the lineages that form the central complex, and will facilitate future studies that address the structure or function of the central complex at the single cell level.
大多数中枢复合体神经元属于 10 个次级（幼虫产生）谱系。在晚期幼虫中，这些谱系的未分化轴突束形成一个原基，其中中枢复合体的所有隔室都可以被识别为离散的实体。四个后级谱系（DPMm1、DPMpm1、DPMpm2 和 CM4）产生了连接protocerebral 桥、扇形体、结节和椭圆体的小场神经元的类群。三个位于前脑的谱系，DALv2、BAmv1 和 DALcl2，分别形成了椭圆体和扇形体的大场神经元。这些谱系为来自视上核的输入通道提供了一个连接，将中枢复合体与相邻的前脑隔室连接起来。后皮质的三个谱系，CM3、CP2 和 DPMpl2，将后脑神经胶与扇形体的特定层连接起来。尽管中枢复合体的所有隔室都通过上述谱系的轴突束在晚期幼虫脑中预先形成，但神经胶在蛹期的前 2 天中分化，此时次级神经元的末端分支和突触形成。在这个阶段，中枢复合体的最初的直水平层在额平面上弯曲，这产生了扇形和椭圆体的特征形状。我们的分析提供了形成中枢复合体的谱系的全面描述，并将有助于未来在单细胞水平上研究中枢复合体的结构或功能的研究。
