Howard Hughes Medical Institute, Janelia Farm Research Campus, 19700 Helix Drive, Ashburn, VA, USA.
Department of Neurobiology, University of Massachusetts, 364 Plantation Street, Worcester, MA, USA.
J Comp Neurol. 2013 Aug 15;521(12):2645-Spc1. doi: 10.1002/cne.23339.
The Drosophila central brain develops from a fixed number of neuroblasts. Each neuroblast makes a clone of neurons that exhibit common trajectories. Here we identified 15 distinct clones that carry larval-born neurons innervating the Drosophila central complex (CX), which consists of four midline structures including the protocerebral bridge (PB), fan-shaped body (FB), ellipsoid body (EB), and noduli (NO). Clonal analysis revealed that the small-field CX neurons, which establish intricate projections across different CX substructures, exist in four isomorphic groups that respectively derive from four complex posterior asense-negative lineages. In terms of the region-characteristic large-field CX neurons, we found that two lineages make PB neurons, 10 lineages produce FB neurons, three lineages generate EB neurons, and two lineages yield NO neurons. The diverse FB developmental origins reflect the discrete input pathways for different FB subcompartments. Clonal analysis enlightens both development and anatomy of the insect locomotor control center.
果蝇的中枢脑由固定数量的神经母细胞发育而来。每个神经母细胞都会产生一个具有共同轨迹的神经元克隆。在这里，我们鉴定了 15 个不同的克隆，它们携带幼虫出生的神经元，这些神经元支配果蝇中枢复合体（CX），包括四个中线结构，包括原脑桥（PB）、扇形体（FB）、椭圆体（EB）和结节（NO）。克隆分析显示，小视野 CX 神经元存在于四个同构的组中，这些神经元通过不同的 CX 亚结构建立复杂的投射，分别来自四个复杂的后 ASE 阴性谱系。就具有区域特征的大视野 CX 神经元而言，我们发现两个谱系产生 PB 神经元，10 个谱系产生 FB 神经元，三个谱系产生 EB 神经元，两个谱系产生 NO 神经元。不同的 FB 发育起源反映了不同 FB 亚区的离散输入途径。克隆分析揭示了昆虫运动控制中心的发育和解剖学。
