College of Chemistry, Jilin University, Changchun, P. R. China.
J Sep Sci. 2011 Jan;34(2):142-9. doi: 10.1002/jssc.201000365. Epub 2010 Dec 7.
A new method was developed for the determination of six fluoroquinolone antibiotics including fleroxacin, levofloxacin, ciprofloxacin, lomfloxacin, enrofloxacin, and sparfloxacin in chicken breast muscle, in which the extraction and clean-up were performed in one step by microwave irradiation. The mixture of ACN containing 0.3% v/v phosphoric acid/water pH 3 (70:30, v/v) was used as the extraction solution and hexane was used as the clean-up solution. The extract was analyzed by liquid chromatography-tandem mass spectrometry system. The RSDs of intra- and inter-day obtained are in the range of 1.0-10.4 and 3.8-13.6%, respectively. In the three fortified levels of chicken breast muscle (20, 100, and 500 ng/g), the recoveries of fluoroquinolone antibiotics ranging from 66.0 to 97.2% are obtained. The LODs are in the range of 2.7-6.7 ng/g. This method simplifies the process of the sample preparation and reduces the operation errors.
建立了一种新的方法，用于测定鸡肉中 6 种氟喹诺酮类抗生素，包括氟罗沙星、左氧氟沙星、环丙沙星、洛美沙星、恩诺沙星和司帕沙星。该方法采用微波辐射，一步提取和净化。提取液为含 0.3%（v/v）磷酸的乙腈/水（pH 3，70:30，v/v）溶液，正己烷作为净化液。采用液相色谱-串联质谱系统进行分析。该方法的日内和日间精密度的 RSD 分别在 1.0-10.4%和 3.8-13.6%范围内。在鸡肉中 3 个加标水平（20、100 和 500ng/g）下，氟喹诺酮类抗生素的回收率在 66.0-97.2%之间。检出限在 2.7-6.7ng/g 之间。该方法简化了样品前处理过程，减少了操作误差。
