[骨碎补总黄酮对兔骨髓干细胞活性及钙沉积的影响]
[Effect of assemble flavone of Drynariae Rhizome on activity and calcium deposition of bone marrow stem cells of rabbit].
作者信息
Shu Xiaochun, Hu Xiangyang, Zhang Lijuan
机构信息
Fifth Affiliated Hospital of Sun Yetsen University, Zhuhai 519000, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2010 Oct;35(20):2760-3.
OBJECTIVE
To observe the effect of assemble flavone of Drynariae Rhizome on the activity and osteogenic differentiation of bone marrow stem cells of rabbit in vitro.
METHOD
Density gradient centrifugation was used to isolate bone marrow stem cells from SD rats. The MSCs were also undergone osteogenic induction. Cytochemical and immunocytochemical tainting were performed to verify their multipotential.
RESULT
High purity of bone marrow stem cells was successfully obtained. Both ALP activity and mineralization nodes tainting were positive in MSCs after osteogenic differentiation.
CONCLUSION
The MSCs sustained their osteogenic differentiation potential in vitro by assemble flavone of rhizome drynaria.
目的
观察骨碎补总黄酮对兔骨髓干细胞活性及体外成骨分化的影响。
方法
采用密度梯度离心法从SD大鼠分离骨髓干细胞，并对其进行成骨诱导。进行细胞化学和免疫细胞化学染色以验证其多能性。
结果
成功获得高纯度的骨髓干细胞。成骨分化后的骨髓干细胞碱性磷酸酶（ALP）活性及矿化结节染色均为阳性。
结论
骨碎补总黄酮可使骨髓干细胞在体外维持其成骨分化潜能。
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