Hassan Amaal Hassan M, Mahmoud Salim, El-Hamidy A
Department of Zoology, Faculty of Science, Sohag University, Jeddah, Saudi Arabia.
J Egypt Soc Parasitol. 2010 Aug;40(2):303-10.
Laboratory investigations were carried out to quantify the amount of total proteins, carbohydrates and reducing sugar in the hemolymph and Digestive Gland Gonad- complex (DGG) of infected and uinfected lanistes arinatus. Snails were naturally infected with two different types of trematode la:val stages (rediae of gymnocepahalus cercaria and sporocyst of xiphidiocercaria), collected from the River Nile at Sohag governorate, Egypt. Analysis was carried out using an extraction of the DGGs tissue with buffer solution, while snails' hemolymph was applied directly. The results revealed that snail infection by rediae of gymnocephalus cercariae led to non significant increasing in both total carbohydrates and protein in hemolymph. However, infection by sporocysts of xiphidiocercariae caused a significant increasing only in hemolymph total protein. On the other hand, the amount of both reducing sugar and total proteins in DGG did not increase significantly whenever the infection caused by both types of trematode larvae. However, total carbohydrates in DGG increased significantly.
进行了实验室研究，以量化感染和未感染的阿里纳特斯蜗牛血淋巴以及消化腺性腺复合体（DGG）中总蛋白、碳水化合物和还原糖的含量。蜗牛自然感染了两种不同类型的吸虫幼虫阶段：从埃及索哈杰省尼罗河采集的裸头蚴雷蚴和剑尾蚴孢蚴。使用缓冲溶液提取DGG组织进行分析，而蜗牛的血淋巴则直接进行检测。结果显示，裸头蚴雷蚴感染蜗牛导致血淋巴中总碳水化合物和蛋白质均无显著增加。然而，剑尾蚴孢蚴感染仅使血淋巴总蛋白显著增加。另一方面，无论由两种吸虫幼虫中的哪一种引起感染，DGG中还原糖和总蛋白的含量均未显著增加。不过，DGG中的总碳水化合物显著增加。
