Sánchez-Céspedes R, Millán Y, Guil-Luna S, García-Monterde J, Reymundo C, Espinosa de los Monteros A, Martín de las Mulas J
Department of Comparative Pathology, Veterinary Faculty, University of Córdoba, Córdoba, Spain.
J Comp Pathol. 2011 Jul;145(1):25-30. doi: 10.1016/j.jcpa.2010.11.006. Epub 2011 Jan 17.
The aim of this study was to determine whether the myoepithelial (ME) cell marker calponin could be used to analyze the integrity of the ME cell layer as a means of identifying canine mammary carcinoma in situ. Tissue from 74 canine mammary lesions was evaluated (two dysplasia, eight benign tumours and 64 carcinomas including one carcinoma in situ). The 63 carcinomas included examples of histological grade 1 (n=32), grade 2 (n=23) and grade 3 (n=8). Expression of calponin was determined by immunohistochemistry. The percentage of proliferating cells surrounded by a single layer of calponin-positive cells formed the basis of classification as type I (≥ 90%), type II (70-90%) and type III (≤ 70%). Expression of Ki67 was used to determine the proliferation index (PI). The malignant tumours comprised of an approximately equal mixture of type I, II and III lesions. The two examples of dysplasia, the carcinoma in situ and two thirds of the benign tumours were classified as type I lesions. Some overlap in the level of calponin expression was observed between benign and malignant tumours. Positive correlations between the degree of calponin expression and the type of lesion (i.e. benign versus malignant; R=+0.3, P=0.08) and the histological grade of malignancy (R=+0.54, P=0.000001) were found. A negative correlation between the degree of calponin expression and PI (R=+0.027, P=0.016) was found. The ME cell marker calponin may be used as an aid in the identification of canine carcinoma in situ, but the study of the ME cell layer integrity is not definitive for the diagnosis of malignancy in canine mammary tumours.
本研究的目的是确定肌上皮（ME）细胞标志物钙调蛋白是否可用于分析ME细胞层的完整性，以此作为识别犬原位乳腺癌的一种方法。对74例犬乳腺病变组织进行了评估（2例发育异常、8例良性肿瘤和64例癌，其中包括1例原位癌）。63例癌包括组织学1级（n = 32）、2级（n = 23）和3级（n = 8）的病例。通过免疫组织化学确定钙调蛋白的表达。被单层钙调蛋白阳性细胞包围的增殖细胞百分比构成了分类为I型（≥90%）、II型（70 - 90%）和III型（≤70%）的基础。使用Ki67的表达来确定增殖指数（PI）。恶性肿瘤由I型、II型和III型病变大致相等的混合组成。2例发育异常、原位癌和三分之二的良性肿瘤被分类为I型病变。在良性和恶性肿瘤之间观察到钙调蛋白表达水平存在一些重叠。发现钙调蛋白表达程度与病变类型（即良性与恶性；R = +0.3，P = 0.08）和恶性组织学分级（R = +0.54，P = 0.000001）之间存在正相关。发现钙调蛋白表达程度与PI之间存在负相关（R = +0.027，P = 0.016）。ME细胞标志物钙调蛋白可用于辅助识别犬原位癌，但对ME细胞层完整性的研究对于犬乳腺肿瘤恶性程度的诊断并不具有决定性意义。
