小鼠 TMJ 加载导致增殖增加和软骨细胞成熟。
Murine TMJ loading causes increased proliferation and chondrocyte maturation.
机构信息
Department of Craniofacial Sciences, Division of Orthodontics, University of Connecticut Health Center, School of Dental Medicine, 263 Farmington Avenue, Farmington, CT 06030, USA.
出版信息
J Dent Res. 2011 Apr;90(4):512-6. doi: 10.1177/0022034510390810. Epub 2011 Jan 19.
Abstract
The purpose of this study was to examine the effects of forced mouth opening on murine mandibular condylar head remodeling. We hypothesized that forced mouth opening would cause an anabolic response in the mandibular condylar cartilage. Six-week-old female C57BL/6 mice were divided into 3 groups: (1) control, (2) 0.25 N, and (3) 0.50 N of forced mouth opening. Gene expression, micro-CT, and proliferation were analyzed. 0.5 N of forced mouth opening caused a significant increase in mRNA expression of Pthrp, Sox9, and Collagen2a1, a significant increase in proliferation, and a significant increase in trabecular spacing in the subchondral bone, whereas 0.25 N of forced mouth opening did not cause any significant changes in any of the parameters examined. Forced mouth opening causes an increase in the expression of chondrocyte maturation markers and an increase in subchondral trabecular spacing.
摘要
本研究旨在探讨强制张口对小鼠下颌髁突头重塑的影响。我们假设强制张口会引起髁突软骨的合成代谢反应。将 6 周龄雌性 C57BL/6 小鼠分为 3 组：（1）对照组，（2）0.25N 和（3）0.50N 的强制张口。分析基因表达、微 CT 和增殖情况。0.5N 的强制张口导致 Pthrp、Sox9 和 Collagen2a1 的 mRNA 表达显著增加、增殖显著增加以及软骨下骨小梁间距显著增加，而 0.25N 的强制张口则未引起任何参数的显著变化。强制张口导致软骨细胞成熟标志物的表达增加和软骨下小梁间距增加。
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