Findley H W, Nasr S, Afify Z, Hnath R, Waldrep K, Ragab A H
Division of Pediatric Hematology/Oncology, Emory University, Atlanta, Georgia.
Cancer Invest. 1990;8(5):493-500. doi: 10.3109/07357909009012073.
In order to determine if recombinant interferon-gamma (rIFN-gamma) can augment the effect of recombinant interleukin-2 (rIL-2) in generating lymphokine-activated killer (LAK) cells, we have incubated normal peripheral blood mononuclear cells (PBMC) with these lymphokines for 3 days and then tested their LAK and natural killer (NK) cell activity. We have found that LAK activity in PBMC from 13 out of 13 normal donors was increased by the combined lymphokines above that due to either lymphokine alone, provided that rIL-2 was present at suboptimal concentration: Optimal levels of rIFN-gamma (100 U/ml) were able to enhance the LAK-inducing activity of suboptimal levels (5 U/ml) but not optimal levels (100 U/ml) of rIL-2. NK activity showed a similar response to these concentrations of lymphokines. Activation of LAK/NK cells was accompanied by increases in the percentages of Leu 19+ (CD56) cells and TAC+ (IL-2-receptor) cells, and in the intensity of TAC antigen expression. These results indicate that combination rIFN-gamma and rIL-2 may be more effective in generating LAK/NK cells than rIL-2 alone, particularly with suboptimal concentrations of rIL-2 such as occur during continuous infusion therapy with this agent.
为了确定重组干扰素-γ（rIFN-γ）是否能增强重组白细胞介素-2（rIL-2）在产生淋巴因子激活的杀伤细胞（LAK细胞）方面的作用，我们将正常外周血单个核细胞（PBMC）与这些淋巴因子一起孵育3天，然后检测它们的LAK和自然杀伤（NK）细胞活性。我们发现，在rIL-2处于次优浓度的情况下，13名正常供体的PBMC中的LAK活性因联合使用上述淋巴因子而高于单独使用任何一种淋巴因子时的活性：最佳水平的rIFN-γ（100 U/ml）能够增强次优水平（5 U/ml）而非最佳水平（100 U/ml）的rIL-2诱导LAK的活性。NK活性对这些浓度的淋巴因子表现出类似的反应。LAK/NK细胞的激活伴随着Leu 19+（CD56）细胞和TAC+（IL-2受体）细胞百分比的增加以及TAC抗原表达强度的增加。这些结果表明，联合使用rIFN-γ和rIL-2在产生LAK/NK细胞方面可能比单独使用rIL-2更有效，特别是在使用该药物进行持续输注治疗时出现的次优浓度的rIL-2情况下。
