Bartelt-Kirbach Britta, Langer-Fischer Katharina, Golenhofen Nikola
Institute of Anatomy and Cell Biology, University of Ulm, Ulm, Germany.
Cell Commun Adhes. 2010 Aug;17(4-6):75-82. doi: 10.3109/15419061.2010.549977. Epub 2011 Jan 21.
Cadherin-mediated specific cell adhesion is an important process in brain development as well as in synaptic plasticity in the adult brain. In this study the authors quantified mRNA levels of N-cadherin and cadherin-11 in different brain regions for the first time. In hippocampus N-cadherin mRNA levels were very high at embryonic stages and decreased during further development, whereas cadherin-11 mRNA levels were highest at postnatal stages. However, N-cadherin protein level was not altered during hippocampal development and cadherin-11 protein was low at embryonic but high at postnatal and adult stages. In cultured hippocampal neurons both cadherins became colocalized and recruited to synaptic sites during ongoing differentiation, with especially high accumulation of cadherin-11 at synapses. These data hint at a critical role of N-cadherin at early embryonic stages and early synaptogenesis, whereas cadherin-11 might be more important for further stabilization of synapses in the postnatal period and adulthood.
钙黏蛋白介导的特异性细胞黏附是大脑发育以及成人大脑突触可塑性中的一个重要过程。在本研究中，作者首次量化了不同脑区中N-钙黏蛋白和钙黏蛋白-11的mRNA水平。在海马体中，N-钙黏蛋白mRNA水平在胚胎阶段非常高，并在进一步发育过程中下降，而钙黏蛋白-11 mRNA水平在出生后阶段最高。然而，N-钙黏蛋白蛋白水平在海马体发育过程中没有改变，钙黏蛋白-11蛋白在胚胎期较低，但在出生后和成年期较高。在培养的海马神经元中，两种钙黏蛋白在持续分化过程中共同定位并募集到突触部位，尤其是钙黏蛋白-11在突触处有特别高的积累。这些数据表明，N-钙黏蛋白在胚胎早期阶段和早期突触形成中起关键作用，而钙黏蛋白-11可能对出生后和成年期突触的进一步稳定更为重要。
