Mohan Raj A B, Gregory N G
AFRC Institute of Food Research, Bristol Laboratory, Langford.
Res Vet Sci. 1990 Nov;49(3):364-6.
Three hundred and twenty broilers were stunned for 2 minutes in batches of 10 per crate in four treatments: 45 per cent carbon dioxide; 55 per cent carbon dioxide; 2 per cent oxygen, or 5 per cent oxygen presented as air diluted by argon. The number of survivors and the time to resumption of consciousness were recorded using the time to eye opening and response to comb pinching as indicators of consciousness. In 45 per cent and 55 per cent carbon dioxide and 2 per cent oxygen, 28, none and eight birds, respectively, survived out of 100 in each group. In 5 per cent oxygen the birds were still fully conscious after a 2 minute exposure period, and the test was discontinued. The time to recovery could be rapid after stunning in both the gases. It is suggested that a concentration of 55 per cent carbon dioxide or less than 1 per cent oxygen are required to kill broilers within a 2 minute exposure period, and that care should be taken to ensure that there is sufficient turbulence within the stunning chamber to avoid air pockets being trapped between the birds.
320只肉鸡被分批装入板条箱，每箱10只，进行2分钟的致昏处理，共有四种处理方式：45%二氧化碳；55%二氧化碳；2%氧气，或5%氧气（以氩气稀释的空气形式呈现）。以睁眼时间和对鸡冠夹捏的反应作为意识指标，记录存活鸡的数量以及恢复意识所需的时间。在45%二氧化碳、55%二氧化碳和2%氧气处理组中，每组100只鸡分别有28只、0只和8只存活。在5%氧气处理组中，鸡在暴露2分钟后仍完全清醒，试验终止。两种气体致昏后恢复时间可能很快。研究表明，在2分钟的暴露期内，杀死肉鸡需要55%的二氧化碳浓度或低于1%的氧气浓度，并且应注意确保致昏室内有足够的湍流，以避免鸡之间 trapped pockets of air。 （原文最后一句“air pockets being trapped between the birds”翻译得有些生硬，推测“trapped”在这里意思不太明确，也许是“困住”之类的意思，你可根据实际情况进一步调整。）
