Molecular Medicine, Deparment of Experimental Medicine Istituto Giannina Gaslini, Largo G. Gaslini 5, 16147 Genoa, Italy.
Diagn Microbiol Infect Dis. 2011 Feb;69(2):213-7. doi: 10.1016/j.diagmicrobio.2010.08.015.
To analyze 67 clinical methicillin-resistant Staphylococcus aureus (MRSA) isolated from pediatric hospital infections, we used multilocus variable-number tandem-repeat DNA sequence-based techniques, targeting the protein A polymorphic X region and the clumping factor B complete R domain. We define a "clfB similarity score" and then compare the double loci analysis of closely related MRSA isolates with pulsed-field gel electrophoresis (PFGE). We found an endemic clone (MLST-ST8, spa-t008, SCCmecIV, ClfB lineage 1) able to originate 3 possible outbreaks and a second clone (MLST-ST152, spa-t355, SCCmecV, ClfB lineage 4) responsible for limited cases of MRSA infections, indicating that the combination of spa and clfB-lineage typing is useful to trace MRSA pediatric outbreaks.
为了分析 67 株从儿科医院感染中分离出的耐甲氧西林金黄色葡萄球菌（MRSA），我们采用了多位点可变数目串联重复 DNA 序列为基础的技术，针对蛋白 A 多态性 X 区和聚集因子 B 完整 R 区。我们定义了一个“clfB 相似评分”，然后将密切相关的 MRSA 分离株的双基因座分析与脉冲场凝胶电泳（PFGE）进行比较。我们发现了一个地方性克隆（MLST-ST8、spa-t008、SCCmecIV、ClfB 谱系 1），能够引发 3 次可能的暴发，以及第二个克隆（MLST-ST152、spa-t355、SCCmecV、ClfB 谱系 4），导致有限数量的 MRSA 感染，表明 spa 和 clfB 谱系分型的结合可用于追踪 MRSA 儿科暴发。
