Wedrychowicz H, Bezubik B
Department of Parasitology, Zoological Institute, University of Warszawa, Poland.
Vet Parasitol. 1990 Nov;37(3-4):273-84. doi: 10.1016/0304-4017(90)90010-9.
Adult New Zealand rabbits were vaccinated at 2-week intervals with three doses of 100 micrograms of infective larval somatic proteins (L3SP) administered subcutaneously with Freund's complete adjuvant (FCA) or beryllium hydroxide and then challenged orally with 10,000 L3. Groups of rabbits immunized orally with three doses of 5000 or 2000 L3 served as vaccination controls. Intestinal worm burdens on Day 21 after challenge revealed that beryllium hydroxide effectively potentiates the protective immunogenicity of L3SP. The level of protection obtained using the beryllium adjuvant (94.8%) was nearly as high as that in rabbits immunized with three doses of 5000 L3 (99.8%). Rabbits vaccinated using FCA showed very poor immunity (29.5%). Local and systemic antibody levels detected by radioimmunoprecipitation tests using 125I-L3SP showed very little correlation with the degree of protection. The beryllium hydroxide-treated group demonstrated significantly higher bile IgA antibody levels than other experimental rabbits. FCA-treated rabbits developed a much higher serum precipitating antibody response, detectable using gel double diffusion tests, than the beryllium group. Also, mucosal IgA antibody levels detected on Day 21 after challenge were significantly higher in the FCA group than in other groups.
成年新西兰兔每隔两周皮下注射三剂100微克感染性幼虫体蛋白（L3SP），佐剂为弗氏完全佐剂（FCA）或氢氧化铍，之后口服10000条L3进行攻毒。口服三剂5000条或2000条L3的兔组作为疫苗接种对照。攻毒后第21天的肠道蠕虫负荷显示，氢氧化铍可有效增强L3SP的保护性免疫原性。使用氢氧化铍佐剂获得的保护水平（94.8%）几乎与接种三剂5000条L3的兔（99.8%）一样高。使用FCA接种的兔免疫力很差（29.5%）。使用125I-L3SP通过放射免疫沉淀试验检测的局部和全身抗体水平与保护程度几乎没有相关性。氢氧化铍处理组的胆汁IgA抗体水平显著高于其他实验兔。FCA处理的兔产生的血清沉淀抗体反应比铍组高得多，可通过凝胶双向扩散试验检测到。此外，攻毒后第21天检测到的FCA组黏膜IgA抗体水平显著高于其他组。
