Water Research Centre, Veolia Environnement, chemin de la Digue, BP 76, 78603, Maisons-Laffitte, France.
Water Sci Technol. 2011;63(2):255-61. doi: 10.2166/wst.2011.046.
The determination of the virus retention capabilities of UF units during operation is essential for the operators of drinking water treatment facilities in order to guarantee an efficient and stable removal of viruses through time. In previous studies, an effective method (MS2-phage challenge tests) was developed by the Water Research Center of Veolia Environnement for the measurement of the virus retention rates (Log Removal Rate, LRV) of commercially available hollow fiber membranes at lab scale. In the present work, the protocol for monitoring membrane performance was transferred from lab scale to pilot scale. Membrane performances were evaluated during pilot trial and compared to the results obtained at lab scale with fibers taken from the pilot plant modules. PFU culture method was compared to RT-PCR method for the calculation of LRV in both cases. Preliminary tests at lab scale showed that both methods can be used interchangeably. For tests conducted on virgin membrane, a good consistency was observed between lab and pilot scale results with the two analytical methods used. This work intends to show that a reliable determination of the membranes performances based on RT-PCR analytical method can be achieved during the operation of the UF units.
为了保证通过时间有效地稳定去除病毒，饮用水处理设施的操作人员必须确定 UF 装置在运行过程中的病毒保留能力。在以前的研究中，威立雅环境水务研究中心开发了一种有效的方法（MS2 噬菌体挑战测试），用于测量市售中空纤维膜在实验室规模下的病毒去除率（对数去除率，LRV）。在本工作中，监测膜性能的方案从实验室规模转移到了中试规模。在中试试验期间评估了膜性能，并将从中试工厂模块中取出的纤维在实验室规模下获得的结果进行了比较。在这两种情况下，PFU 培养法和 RT-PCR 法均用于计算 LRV。实验室规模的初步测试表明，这两种方法可以互换使用。对于新膜的测试，两种分析方法在实验室和中试规模的结果之间观察到良好的一致性。本工作旨在表明，在 UF 装置的运行过程中，可以基于 RT-PCR 分析方法可靠地确定膜的性能。
