Zou W Z, Zhou K X
Department of Pathology, Beijing Medical University.
Chin Med J (Engl). 1990 Oct;103(10):805-10.
A model of crescentic glomerulonephritis (GN) in mice was induced by intravenous injection of rabbit anti-mouse glomerular basement membrane (GBM) antiserum and lipopolysaccharide. The procedure was carried out in BALB/c mice, heterozygous mice and nude mice. In order to examine the role of T cells in the pathogenesis of crescentic GN, immunofluorescent and morphologic changes in the glomeruli of these animals were studied. Intense (4+) linear deposition of rabbit IgG was found along the GBM of all test animals. Intense (3(+)-4+) linear deposition of mouse IgG along the GBM was present in normal and heterozygous mice, but not in nude mice. Normal mice developed typical crescentic GN characterized by severe degeneration and destruction of GBM, fibrin deposition and crescent formation 3-6 weeks after injection. Heterozygous mice only developed mild mesangial proliferation. No glomerular lesions were seen in nude mice. These preliminary data suggest that glomerular immunologic damage requires the participation of functional T cells, and that the induction of typical crescentic GN requires integral T-cell function.
通过静脉注射兔抗小鼠肾小球基底膜（GBM）抗血清和脂多糖诱导小鼠新月体性肾小球肾炎（GN）模型。该实验在BALB/c小鼠、杂合子小鼠和裸鼠中进行。为了研究T细胞在新月体性GN发病机制中的作用，对这些动物肾小球的免疫荧光和形态学变化进行了研究。在所有受试动物的GBM上均发现兔IgG呈强（4+）线性沉积。正常小鼠和杂合子小鼠的GBM上存在小鼠IgG强（3+-4+）线性沉积，而裸鼠则没有。正常小鼠在注射后3-6周出现典型的新月体性GN，其特征为GBM严重变性和破坏、纤维蛋白沉积和新月体形成。杂合子小鼠仅出现轻度系膜增生。裸鼠未见肾小球病变。这些初步数据表明，肾小球免疫损伤需要功能性T细胞的参与，典型新月体性GN的诱导需要完整的T细胞功能。
