Hayzer D J, Young-Cooper G O, Mage R G, Jaton J C
Department of Medical Biochemistry, Medical Center of the University of Geneva, Switzerland.
Eur J Immunol. 1990 Dec;20(12):2707-12. doi: 10.1002/eji.1830201227.
A cDNA library derived from spleen cells of an unimmunized rabbit expressing the c7 phenotype of Ig lambda chains (c7+, c21-) was screened with V lambda or C lambda probes of a lambda light chain bearing c21 epitopes. The nucleotide sequences of three hybridizing clones were found to be identical within the V lambda, J lambda and C lambda regions. The V lambda region was 97% similar to that of the functional germ-line gene V lambda 2, and the C lambda region was identical to that of gene C lambda 6, recently identified. Gene C lambda 6 exhibited four codon differences when compared with gene C lambda 5, the latter encoding c21 epitopes. The data presented here and in the accompanying report (Jaton, J.-C. et al., Eur. J. Immunol. 1990, 20:2713) support the view that gene C lambda 6 encodes the C region of c7 lambda chains and that c7 and c21 markers designate two distinct isotypic forms of lambda chains. On the basis of comparative Southern blotting analyses and restriction maps of cloned genomic regions containing V lambda and C lambda genes, a scheme is proposed to account for the c7- and c21- phenotypes.
用带有c21表位的λ轻链的Vλ或Cλ探针筛选来自未免疫的、表达Igλ链c7表型（c7 +，c21 -）的兔脾细胞的cDNA文库。发现三个杂交克隆在Vλ、Jλ和Cλ区域内的核苷酸序列相同。Vλ区域与功能性种系基因Vλ2的Vλ区域有97%的相似性，Cλ区域与最近鉴定的基因Cλ6的Cλ区域相同。与编码c21表位的基因Cλ5相比，基因Cλ6表现出四个密码子差异。本文及随附报告（Jaton，J.-C.等人，《欧洲免疫学杂志》，1990年，20:2713）中的数据支持以下观点：基因Cλ6编码c7λ链的C区域，并且c7和c21标记表示λ链的两种不同的同种型形式。基于对包含Vλ和Cλ基因的克隆基因组区域的比较Southern印迹分析和限制性图谱，提出了一个解释c7和c21表型的方案。
