果蝇光感受器顶域调节中中心体蛋白和 bazooka 的遗传相互作用
Genetic interaction of centrosomin and bazooka in apical domain regulation in Drosophila photoreceptor.
机构信息
Department of Biology, Baylor University, Waco, Texas, United States of America.
出版信息
PLoS One. 2011 Jan 6;6(1):e16127. doi: 10.1371/journal.pone.0016127.
BACKGROUND
Cell polarity genes including Crumbs (Crb) and Par complexes are essential for controlling photoreceptor morphogenesis. Among the Crb and Par complexes, Bazooka (Baz, Par-3 homolog) acts as a nodal component for other cell polarity proteins. Therefore, finding other genes interacting with Baz will help us to understand the cell polarity genes' role in photoreceptor morphogenesis.
METHODOLOGY/PRINCIPAL FINDINGS: Here, we have found a genetic interaction between baz and centrosomin (cnn). Cnn is a core protein for centrosome which is a major microtubule-organizing center. We analyzed the effect of the cnn mutation on developing eyes to determine its role in photoreceptor morphogenesis. We found that Cnn is dispensable for retinal differentiation in eye imaginal discs during the larval stage. However, photoreceptors deficient in Cnn display dramatic morphogenesis defects including the mislocalization of Crumbs (Crb) and Bazooka (Baz) during mid-stage pupal eye development, suggesting that Cnn is specifically required for photoreceptor morphogenesis during pupal eye development. This role of Cnn in apical domain modulation was further supported by Cnn's gain-of-function phenotype. Cnn overexpression in photoreceptors caused the expansion of the apical Crb membrane domain, Baz and adherens junctions (AJs).
CONCLUSIONS/SIGNIFICANCE: These results strongly suggest that the interaction of Baz and Cnn is essential for apical domain and AJ modulation during photoreceptor morphogenesis, but not for the initial photoreceptor differentiation in the Drosophila photoreceptor.
背景
细胞极性基因，包括 Crumbs（Crb）和 Par 复合物，对于控制光感受器形态发生是必不可少的。在 Crb 和 Par 复合物中，Bazooka（Baz，Par-3 同源物）作为其他细胞极性蛋白的节点成分发挥作用。因此，找到与 Baz 相互作用的其他基因将有助于我们理解细胞极性基因在光感受器形态发生中的作用。
方法/主要发现：在这里，我们发现了 baz 和 centrosomin（cnn）之间的遗传相互作用。Cnn 是中心体的核心蛋白，中心体是主要的微管组织中心。我们分析了 cnn 突变对发育中的眼睛的影响，以确定其在光感受器形态发生中的作用。我们发现，在幼虫阶段，Cnn 对于眼盘发育中的视网膜分化不是必需的。然而，cnn 缺陷的光感受器显示出明显的形态发生缺陷，包括 Crb 和 Bazooka（Baz）在中龄蛹眼发育过程中的定位错误，表明 Cnn 专门需要在蛹眼发育过程中用于光感受器形态发生。Cnn 在顶端域调节中的作用进一步得到了 Cnn 的功能获得表型的支持。在光感受器中过表达 Cnn 导致顶端 Crb 膜域、Baz 和黏着连接（AJs）的扩张。
结论/意义：这些结果强烈表明，Baz 和 Cnn 的相互作用对于光感受器形态发生中的顶端域和 AJ 调节是必不可少的，但对于果蝇光感受器中的初始光感受器分化不是必需的。
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