通过引物竞争聚合酶链反应对 mdx、mdx3cv 和 mdx4cv 小鼠进行基因分型。
Genotyping mdx, mdx3cv, and mdx4cv mice by primer competition polymerase chain reaction.
机构信息
Department of Molecular Microbiology and Immunology, University of Missouri School of Medicine, Columbia, MO 65212, USA.
出版信息
Muscle Nerve. 2011 Feb;43(2):283-6. doi: 10.1002/mus.21873. Epub 2010 Dec 9.
Abstract
mdx, mdx3cv, and mdx4cv mice are among the most commonly used models for the study of Duchenne muscular dystrophy. Their disease is caused by point mutations in the dystrophin gene. Despite widespread use of these models, genotyping has not always been straightforward. Current methods require multiple polymerase chain reactions (PCRs), post-PCR manipulations, and/or special equipment/reagents. Herein we report a simple, robust PCR genotyping method based on primer competition. This approach could also be applied in genotyping other point-mutation models.
摘要
mdx、mdx3cv 和 mdx4cv 小鼠是研究杜氏肌营养不良症最常用的模型之一。它们的疾病是由 dystrophin 基因突变引起的。尽管这些模型被广泛使用，但基因分型并不总是那么简单。目前的方法需要多次聚合酶链反应（PCR）、PCR 后操作和/或特殊设备/试剂。在此，我们报告了一种基于引物竞争的简单、稳健的 PCR 基因分型方法。这种方法也可以应用于其他点突变模型的基因分型。
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