Daks A A, Deriusheva S E, Krasikova A V, Zlotina A M, Gaginskaia E R, Galkina S A
Genetika. 2010 Oct;46(10):1335-8.
Avian oocyte chromosomes are transfomed into giant transcriptionally active lampbrush chromosomes (LBCs) at meiosis 1 diplotene. These chromosomes are a convenient tool for high-resulution cytogenetic analysis. Using differential staining with fluorochromes DAPI and CMA3, we have constructed detailed cytological maps for lampbrush macrochromosomes 1-5 and ZW of the Japanese quail Coturnix coturnix japonica. We also performed a comparative analysis ofmitotic chromosomes and LBCs corresponding to them. We estimated the decondensation coefficient during LBC formation and determined the centromere indices for mitotic and diplotene chromosomes and thus found that different chromosomes and chromosomal regions demonstrate unequal degrees of decondensation.
鸟类卵母细胞染色体在减数第一次分裂双线期转变为巨大的转录活跃的灯刷染色体（LBCs）。这些染色体是进行高分辨率细胞遗传学分析的便利工具。通过使用荧光染料DAPI和CMA3进行差异染色，我们构建了日本鹌鹑（Coturnix coturnix japonica）灯刷常染色体1-5和ZW的详细细胞学图谱。我们还对有丝分裂染色体及其对应的LBCs进行了比较分析。我们估计了LBC形成过程中的解聚系数，并确定了有丝分裂染色体和双线期染色体的着丝粒指数，从而发现不同的染色体和染色体区域表现出不等程度的解聚。
