Mikhaĭlova E I, Lovtsius A V, Sosnikhina S P
Genetika. 2010 Oct;46(10):1371-5.
The Peterhof Collection of spontaneous meiotic mutants of rye was used as a model to study the genetic control of meiosis key events in an organism with a large genome. A combination of methods, which included fluorescence in situ DNA-DNA hybridization, sequencing of recombinogenic proteins, and immunocytochemical analysis of meiosis proteins, clearly showed that mutation sy1 affects recombination events, asynapsis in mutant sy9 is connected with defects of the assembly of synaptonemal complex axial cores, and that synapsis defects in mutant sy10 are coupled with the presence of protein Zyp1 in the core region. The assembly of proteins Asyl and Zyp1 on the axes of meiotic chromosomes was shown to occur separately, which is a specific feature of rye, as compared to arabidopsis.
以彼得霍夫黑麦自发减数分裂突变体系作为模型，研究大基因组生物体中减数分裂关键事件的遗传控制。综合运用包括荧光原位DNA-DNA杂交、重组蛋白测序以及减数分裂蛋白免疫细胞化学分析在内的多种方法，结果清晰表明，突变体sy1影响重组事件，突变体sy9中的联会紊乱与联会复合体轴向核心组装缺陷有关，而突变体sy10中的联会缺陷则与核心区域存在蛋白Zyp1有关。与拟南芥相比，减数分裂染色体轴上Asyl和Zyp1蛋白的组装是分开进行的，这是黑麦的一个特定特征。
