Max-Planck-Institut für Molekulare Pflanzenphysiologie, Am Mühlenberg 1, Potsdam-Golm, Germany.
J Plant Physiol. 2011 Aug 15;168(12):1452-61. doi: 10.1016/j.jplph.2010.12.009. Epub 2011 Jan 21.
Photosystem I (PSI), the plastocyanin-ferredoxin oxidoreductase of the photosynthetic electron transport chain, is one of the largest bioenergetic complexes known. It is composed of subunits encoded in both the chloroplast genome and the nuclear genome and thus, its assembly requires an intricate coordination of gene expression and intensive communication between the two compartments. In this review, we first briefly describe PSI structure and then focus on recent findings on the role of the two small chloroplast genome-encoded subunits PsaI and PsaJ in the stability and function of PSI in higher plants. We then address the sequence of PSI biogenesis, discuss the role of auxiliary proteins involved in cofactor insertion into the PSI apoproteins and in the establishment of protein-protein interactions during subunit assembly. Finally, we consider potential limiting steps of PSI biogenesis, and how they may contribute to the control of PSI accumulation.
光系统 I（PSI）是光合作用电子传递链中的质体蓝素-铁氧还蛋白氧化还原酶，是已知的最大的生物能复合物之一。它由叶绿体基因组和核基因组编码的亚基组成，因此，其组装需要基因表达的精细协调和两个隔室之间的密集通信。在这篇综述中，我们首先简要描述了 PSI 的结构，然后重点介绍了最近关于两个小的叶绿体基因组编码亚基 PsaI 和 PsaJ 在高等植物 PSI 稳定性和功能中的作用的发现。我们接着讨论了 PSI 生物发生的顺序，讨论了辅助蛋白在将辅助因子插入 PSI 脱辅基蛋白和在亚基组装过程中建立蛋白-蛋白相互作用中的作用。最后，我们考虑了 PSI 生物发生的潜在限制步骤，以及它们如何有助于 PSI 积累的控制。
