Department of Urology, Faculty of Medicine, Hacettepe University, Ankara, Turkey.
Urology. 2011 Mar;77(3):761.e1-7. doi: 10.1016/j.urology.2010.10.009. Epub 2011 Jan 22.
To evaluate the gross morphometric changes and in vitro responses of the corpus cavernosus of rats treated with sildenafil citrate after cavernous neurotomy.
The animals were divided into 3 groups. Group 1 consisted of sham-operated rats (n = 16); group 2 consisted of rats that underwent bilateral cavernous neurotomy (BCN) (n = 16); and group 3 consisted of rats that underwent unilateral cavernous neurotomy (UCN) (n = 16). Each group of rats was further classified into 2 subgroups according to whether or not they received sildenafil treatment. The rats were killed on postoperative day 14, and penectomy was performed. Apoptosis was assessed by terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling (TUNEL), and organ-bath studies were evaluated by Phenylephrine (Phe), acetylcholine (Ach), sodium nitroprusside (SNP), and electrical field stimulation (EFS) responses.
Penile weight in the BCN group was significantly lower than that of sham-treated group. UCN allowed much more preservation of penile weight compared with that in the sham-treated group. Sildenafil citrate treatment had positive effects on penile weight of both BCN (P = .003) and UCN (P = .004) groups. BCN increased smooth muscle apoptosis when compared with the sham or UCN group. Sildenafil citrate had a positive effect on the apoptotic index. In the BCN group, responses to Phe, Ach, SNP, and EFS decreased significantly, and sildenafil treatment corrected the responses to Phe, Ach, and SNP.
Our experimental study results support that early and daily sildenafil citrate treatment has a protective affect on the adrenergic and cholinergic systems, which play a role in erectile function.
评估给予枸橼酸西地那非治疗后行海绵体神经切断术大鼠海绵体的大体形态学变化和体外反应。
动物分为 3 组。第 1 组为假手术大鼠（n=16）；第 2 组为行双侧海绵体神经切断术（BCN）大鼠（n=16）；第 3 组为行单侧海绵体神经切断术（UCN）大鼠（n=16）。每组大鼠根据是否接受西地那非治疗进一步分为 2 个亚组。术后第 14 天处死大鼠，行阴茎切除术。采用末端脱氧核苷酸转移酶介导的脱氧尿苷三磷酸缺口末端标记法（TUNEL）评估凋亡，通过苯肾上腺素（Phe）、乙酰胆碱（Ach）、硝普钠（SNP）和电刺激（EFS）反应评估器官浴研究。
BCN 组的阴茎重量明显低于假手术组。UCN 与假手术组相比，更能保留阴茎重量。枸橼酸西地那非治疗对 BCN（P=0.003）和 UCN（P=0.004）组的阴茎重量均有积极影响。与假手术或 UCN 组相比，BCN 增加了平滑肌凋亡。枸橼酸西地那非对凋亡指数有积极影响。在 BCN 组，Phe、Ach、SNP 和 EFS 的反应明显下降，西地那非治疗纠正了 Phe、Ach 和 SNP 的反应。
我们的实验研究结果支持，早期和每日给予枸橼酸西地那非治疗对在勃起功能中起作用的肾上腺素能和胆碱能系统有保护作用。
