用于测定血清中转铁蛋白结合铁的高效液相免疫亲和色谱法。

A high-performance liquid immunoaffinity chromatography method for determining transferrin-bound iron in serum.

机构信息

Department of Human Development, Kanagawa University of Human Service, Yokosuka, Japan.

出版信息

Clin Chim Acta. 2011 May 12;412(11-12):914-9. doi: 10.1016/j.cca.2011.01.019. Epub 2011 Jan 21.

DOI:10.1016/j.cca.2011.01.019

PMID:21256833

Abstract

BACKGROUND

The analyzed values in the ICSH reference method for serum iron analysis are affected by non-transferrin(Tf)-bound iron such as ferritin. Also, non-Tf-bound plasma iron (iron citrate) is present in iron-overloaded specimens from patients with hemochromatosis, which was measured as serum iron in previous methods. We developed a specific determination method for serum transferrin-bound iron (serum t Fe) by high-performance liquid immunoaffinity chromatography (HPLAC), and compared it with the ICSH method and a fully automated (FA) method.

METHODS

Tf and t Fe were isolated from interferents in serum by HPLC using an immunoaffinity column. The concentration of t Fe isolated was determined by a colorimetric reaction using a highly sensitive chromogen.

RESULTS

Interferents, except iron saccharate (detected at 5%), do not affect t Fe determination. Within-run and between-run imprecisions were in the ranges of 0.2-0.4% and 0.4-1.0% CV. The results of the HPLAC method correlated well with those of the ICSH method (r=0.9993) and FA method (r=0.9984).

CONCLUSIONS

In contrast to the ICSH and FA methods for determining serum iron, the HPLAC method is simple, highly precise and specific for serum t Fe, which can contribute to the measurement of iron status.

摘要

背景

ICSH 血清铁分析参考方法中的分析值受非转铁蛋白（Tf）结合铁（如铁蛋白）的影响。此外，患有血色病的患者的铁过载标本中存在非 Tf 结合血浆铁（柠檬酸铁），在以前的方法中被测量为血清铁。我们开发了一种通过高效液相免疫亲和色谱（HPLAC）测定血清转铁蛋白结合铁（血清 tFe）的特异性测定方法，并将其与 ICSH 方法和全自动（FA）方法进行了比较。