The National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Gene. 2011 Jun 1;478(1-2):28-34. doi: 10.1016/j.gene.2011.01.011. Epub 2011 Jan 21.
TaSnRK2.7, a SnRK2 (sucrose non-fermenting1-related protein kinase 2) member of wheat, confers enhanced multi-stress tolerance in carbohydrate metabolism. Here, the nucleotide diversity of TaSnRK2.7 was investigated in 60 wheat accessions with different stress-tolerant features. One copy, named TaSnRK2.7-B, was detected in 32 accessions, which mainly exhibited stress tolerance. The TaSnRK2.7-B evolutionary dynamics were examined in these cultivars, which have been subjected to population bottlenecks and intensive selection in breeding. Nucleotide diversity (π) in the entire TaSnRK2.7-B region was 0.00148. Sliding-window analysis demonstrated that 3932-4535bp was a variation-enriched region, covering the eighth intron, the ninth exon and the 3'-flanking region. As predicted, neutrality tests revealed that the TaSnRK2.7-B protein was relatively conservative, and population bottlenecks or purifying selection may have acted on TaSnRK2.7-B. Furthermore, linkage disequilibrium between SNP loci extends across the entire TaSnRK2.7-B region. A SNP marker of TaSnRK2.7-B was developed and mapped on chromosome 2AL flanked by WMC179.4 and WMC401, which were co-located in the same or adjacent chromosome intervals with QTLs for phosphorus utilization efficiency and accumulation efficiency of stem water-soluble carbohydrates.
TaSnRK2.7，一个小麦的 SnRK2（蔗糖非发酵相关蛋白激酶 2）成员，在碳水化合物代谢中赋予增强的多胁迫耐受性。在这里，对具有不同耐受力特征的 60 个小麦品种的 TaSnRK2.7 的核苷酸多样性进行了研究。在 32 个品种中检测到一个拷贝，命名为 TaSnRK2.7-B，其主要表现出胁迫耐受性。在这些品种中，对 TaSnRK2.7-B 的进化动态进行了研究，这些品种在育种过程中经历了种群瓶颈和强烈的选择。整个 TaSnRK2.7-B 区域的核苷酸多样性（π）为 0.00148。滑动窗口分析表明，3932-4535bp 是一个变异丰富的区域，包含第八个内含子、第九个外显子和 3'-侧翼区。如预测的那样，中性检验表明 TaSnRK2.7-B 蛋白相对保守，种群瓶颈或纯化选择可能作用于 TaSnRK2.7-B。此外，SNP 位点之间的连锁不平衡延伸到整个 TaSnRK2.7-B 区域。开发了 TaSnRK2.7-B 的 SNP 标记，并将其映射到染色体 2AL 上，由 WMC179.4 和 WMC401 侧翼，它们位于同一或相邻的染色体区间内，与磷利用效率和茎水溶性碳水化合物积累效率的 QTL 共定位。
