Zhang Zhao-Gui, Lv Guang-de, Li Bing, Wang Jia-Jia, Zhao Yan, Kong Fan-Mei, Guo Ying, Li Si-Shen
State Key Laboratory of Crop Biology/Shandong Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, China.
Tai'an Academy of Agricultural Science, Tai'an, China.
PLoS One. 2017 Mar 29;12(3):e0174425. doi: 10.1371/journal.pone.0174425. eCollection 2017.
Sucrose non-fermenting 1-related protein kinases (SnRKs) comprise a major family of signaling genes in plants and are associated with metabolic regulation, nutrient utilization and stress responses. This gene family has been proposed to be involved in sucrose signaling. In the present study, we cloned three copies of the TaSnRK2.10 gene from bread wheat on chromosomes 4A, 4B and 4D. The coding sequence (CDS) is 1086 bp in length and encodes a protein of 361 amino acids that exhibits functional domains shared with SnRK2s. Based on the haplotypes of TaSnRK2.10-4A (Hap-4A-H and Hap-4A-L), a cleaved amplified polymorphic sequence (CAPS) marker designated TaSnRK2.10-4A-CAPS was developed and mapped between the markers D-1092101 and D-100014232 using a set of recombinant inbred lines (RILs). The TaSnRK2.10-4B alleles (Hap-4B-G and Hap-4B-A) were transformed into allele-specific PCR (AS-PCR) markers TaSnRK2.10-4B-AS1 and TaSnRK2.10-4B-AS2, which were located between the markers D-1281577 and S-1862758. No diversity was found for TaSnRK2.10-4D. An association analysis using a natural population consisting of 128 winter wheat varieties in multiple environments showed that the thousand grain weight (TGW) and spike length (SL) of Hap-4A-H were significantly higher than those of Hap-4A-L, but pant height (PH) was significantly lower.
蔗糖非发酵1相关蛋白激酶（SnRKs）是植物中一个主要的信号基因家族，与代谢调控、养分利用和胁迫反应相关。该基因家族被认为参与蔗糖信号传导。在本研究中，我们从普通小麦位于4A、4B和4D染色体上克隆了三个TaSnRK2.10基因拷贝。编码序列（CDS）长度为1086 bp，编码一个361个氨基酸的蛋白质，该蛋白质具有与SnRK2s共享的功能结构域。基于TaSnRK2.10 - 4A的单倍型（Hap - 4A - H和Hap - 4A - L），开发了一个名为TaSnRK2.10 - 4A - CAPS的酶切扩增多态性序列（CAPS）标记，并使用一组重组自交系（RILs）将其定位在标记D - 1092101和D - 100014232之间。TaSnRK2.10 - 4B等位基因（Hap - 4B - G和Hap - 4B - A）被转化为等位基因特异性PCR（AS - PCR）标记TaSnRK2.10 - 4B - AS1和TaSnRK2.10 - 4B - AS2，它们位于标记D - 1281577和S - 1862758之间。未发现TaSnRK2.10 - 4D存在多样性。利用由128个冬小麦品种组成的自然群体在多个环境中进行的关联分析表明，Hap - 4A - H的千粒重（TGW）和穗长（SL）显著高于Hap - 4A - L，但株高（PH）显著低于Hap - 4A - L。
