Pájaro M C, Albesa I
Universidad Nacional de Río Cuarto, Departmento de Microbiología e Inmunología, Río Cuarto, Provincia de Córdoba.
Rev Argent Microbiol. 1990 Jan-Mar;22(1):24-30.
Pyoverdin was purified from Pseudomonas fluorescens cultured in a synthetic medium. Cytotoxic effect on human leukocytes was assayed. Death and lysis was observed depending on concentration and time. Sub-lytic dose decreased leukocyte phagocytosis. The pigment was produced by P. fluorescens in blood stored at 4 degrees C. Saline precipitation of plasma showed that globulin and albumin fraction retained 46% and 37% of pyoverdin, respectively. By means of dialysis it was possible to determine that albumin retained more pigment than the other fractions either bound to the protein or aggregated.
绿脓菌素是从在合成培养基中培养的荧光假单胞菌中纯化得到的。检测了其对人白细胞的细胞毒性作用。观察到细胞死亡和裂解取决于浓度和时间。亚裂解剂量会降低白细胞吞噬作用。该色素由荧光假单胞菌在4℃储存的血液中产生。血浆的盐水沉淀表明球蛋白和白蛋白组分分别保留了46%和37%的绿脓菌素。通过透析可以确定，白蛋白比其他与蛋白质结合或聚集的组分保留了更多的色素。
