鸡和大鼠松果体血清素N - 乙酰基转移酶活性测量的改进方法。

Improved method for the measurement of chicken and rat pineal serotonin N-acetyltransferase activity.

作者信息

Rodriguez-Cabello J C, Arpa F, Agapito M T, Garcia-Herrero I, Recio J M

机构信息

Departamento de Bioquimica, Biologica Molecular y Fisiologia, Facultad de Ciencias, Universidad de Valladolid, Spain.

出版信息

J Pineal Res. 1990;9(2):103-12. doi: 10.1111/j.1600-079x.1990.tb00698.x.

DOI:10.1111/j.1600-079x.1990.tb00698.x

PMID:2126038

Abstract

EGTA, a nonspecific ion chelator, showed very good potential for preventing the loss of serotonin N-acetyl transferase activity (arylamine: acetyl-CoA:N-acetyltransferase, EC 2.3.1.5.) (SNAT) from rat and chicken pineal glands during preincubation at 37 degrees C before substrate addition. SNAT activity was higher than that of the controls when EGTA was present in the homogenization buffer due to the prevention of the loss of activity probably not only during the preincubation period but also during incubation with its substrates. This characteristic of EGTA suggests that its use is suitable in the improved SNAT assay described in this paper since higher SNAT activities were found in both rat and chicken pineal glands than the activities recorded with the usual methods.

摘要

乙二醇双乙醚二胺四乙酸（EGTA），一种非特异性离子螯合剂，在添加底物前于37℃预孵育期间，对于防止大鼠和鸡松果体中血清素N - 乙酰转移酶活性（芳胺：乙酰辅酶A：N - 乙酰转移酶，EC 2.3.1.5.）（SNAT）的丧失显示出很好的潜力。当在匀浆缓冲液中存在EGTA时，SNAT活性高于对照组，这可能是因为EGTA不仅在预孵育期间，而且在与底物孵育期间都防止了活性的丧失。EGTA的这一特性表明，它适用于本文所述的改进的SNAT测定法，因为在大鼠和鸡松果体中发现的SNAT活性均高于用常规方法记录的活性。