Shi Xianzhe, He Zhihui, Dou Abo, Zhang Fengxia, Lian Wenliu, Xu Guowang
Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
Se Pu. 2010 Aug;28(8):765-8. doi: 10.3724/sp.j.1123.2010.00765.
A liquid chromatography-mass spectrometry (LC-MS) method was developed for the metabonomics study of menthol cigarette effect on rats. Urines from three groups of rats were analyzed, including control rats, rats treated with menthol cigarette and rats treated with normal cigarette, and the data were processed by the method of principal component analysis (PCA). The PCA score plot showed that the metabolic difference between the rats treated with menthol cigarette and the control rats was smaller than that between the rats treated with normal cigarette and the control rats. Based on the PCA score plot, eight important metabolites, for example, kynurenic acid, were found and identified. Their relative concentration changes among the three groups further indicated that menthol cigarette maybe decrease the metabolic effect on rats.
建立了一种液相色谱-质谱联用（LC-MS）方法用于薄荷醇香烟对大鼠影响的代谢组学研究。分析了三组大鼠的尿液，包括对照大鼠、用薄荷醇香烟处理的大鼠和用普通香烟处理的大鼠，并采用主成分分析（PCA）方法对数据进行处理。PCA得分图显示，用薄荷醇香烟处理的大鼠与对照大鼠之间的代谢差异小于用普通香烟处理的大鼠与对照大鼠之间的代谢差异。基于PCA得分图，发现并鉴定了8种重要代谢物，例如犬尿烯酸。它们在三组中的相对浓度变化进一步表明，薄荷醇香烟可能会降低对大鼠的代谢影响。
