El'kin A A, Grishko V V, Ivshina I B
Prikl Biokhim Mikrobiol. 2010 Nov-Dec;46(6):637-43.
Comparative study of sulfoxidation activity of free and immobilized Rhodococcus rhodochrous IEGM 66 cells was performed. Free Rhodococcus cells (in the presence of 0.1 vol % n-hexadecane) displayed maximal oxidative activity towards thioanisole (0.5 g/l), a prochiral organic sulfide, added after 48-h cultivation of bacterial cells. Higher sulfide concentrations inhibited sulfoxidation activity of Rhodococcus. Use of immobilized cells allowed the 2-day preparatory stage to be omitted and a complete thioanisole bioconversion to be achieved in 24 h in the case that biocatalyst and 0.5 g/l thioanisole were added simultaneously. The biocatalyst immobilized on gel provides for complete thioanisole transformation into (S)-thioanisole sulfoxide (optical purity of 82.1%) at high (1.0-1.5 g/l) concentrations of sulfide substrate.
对游离和固定化的红平红球菌IEGM 66细胞的亚砜氧化活性进行了比较研究。游离的红球菌细胞（在存在0.1体积%正十六烷的情况下）对硫代苯甲醚（0.5 g/l）表现出最大氧化活性，硫代苯甲醚是一种前手性有机硫化物，在细菌细胞培养48小时后添加。较高的硫化物浓度会抑制红球菌的亚砜氧化活性。使用固定化细胞可以省略为期2天的准备阶段，并且在生物催化剂和0.5 g/l硫代苯甲醚同时添加的情况下，24小时内可实现硫代苯甲醚的完全生物转化。固定在凝胶上的生物催化剂在高（1.0 - 1.5 g/l）浓度的硫化物底物条件下可将硫代苯甲醚完全转化为（S）-硫代苯甲醚亚砜（光学纯度为82.1%）。
