Kelebina T S, Seliakh I O, Gorkovskiĭ A A, Bezsonov E E, El'darov M A, Novak M I, Domracheva A G, Bartoshevich Iu E
Prikl Biokhim Mikrobiol. 2010 Nov-Dec;46(6):666-71.
Alterations of cell walls of Acremonium chrysogenum occurring at intensive synthesis of cephalosporin C has been studied. It is shown, using electron microscopy, that the cell wall of the cells ofATCC 11550 strain ("wild" type) became looser and thicker during growth. The cell wall of the cells of strain 26/8 (hyperautotroph of cephalosporin C) considerably degraded by the end of the stationary phase. Biochemical analysis has shown that these alterations entailed decrease of the proteins' content covalently or noncovalently linked with the polysaccharides of cell walls of both strains. An increase of sensitivity of cell walls of the strain-superproducer to an activity of lytic enzymes of chitinase, laminarinase, proteinase K, and lyticase preparation has been observed during the growth, but this increase has not been found in the case of "wild" type strain. The obtained results evidence to the structure failure of the cell wall of A. chrysogenum entailing the intensive creation of antibiotic.
对产黄青霉在头孢菌素C大量合成时细胞壁的变化进行了研究。通过电子显微镜观察发现，ATCC 11550菌株（“野生”型）细胞的细胞壁在生长过程中变得更疏松且更厚。26/8菌株（头孢菌素C的超自养型）细胞的细胞壁在稳定期末期显著降解。生化分析表明，这些变化导致了与两菌株细胞壁多糖共价或非共价连接的蛋白质含量降低。在生长过程中观察到，高产菌株细胞壁对几丁质酶、海带多糖酶、蛋白酶K和溶菌酶制剂的裂解酶活性的敏感性增加，但“野生”型菌株未出现这种增加。所得结果证明产黄青霉细胞壁结构破坏导致抗生素大量产生。
