Novak M I, Bartoshevich Iu E, Nekliudov A D, Iudina O D, Tsibanov V V
Prikl Biokhim Mikrobiol. 1986 May-Jun;22(3):356-62.
The intracellular and extracellular amino acid composition of an auxotrophic methionine-deficient strain of Acremonium chrysogenum was studied in respect of the content of various carbohydrates in the fermentation medium. In the presence of glucose, an intensive involvement of exogenous DL-methionine into the cell metabolism was observed at earlier stages than in the presence of dextran or succrose. The total content of intracellular amino acids was lower in the cells grown on the medium with glucose. The production of cephalosporin C depended on the intracellular content of methionine, glutamic acid and lysine.
就发酵培养基中各种碳水化合物的含量，研究了产黄顶孢霉营养缺陷型蛋氨酸缺陷菌株的细胞内和细胞外氨基酸组成。与存在葡聚糖或蔗糖的情况相比，在葡萄糖存在下，更早阶段就观察到外源DL-蛋氨酸大量参与细胞代谢。在以葡萄糖为培养基生长的细胞中，细胞内氨基酸的总含量较低。头孢菌素C的产生取决于蛋氨酸、谷氨酸和赖氨酸的细胞内含量。
