Center for Advanced Biomedical Sciences, Waseda University, TWIns, Tokyo, Japan.
Chem Phys Lipids. 2011 Mar;164(3):211-5. doi: 10.1016/j.chemphyslip.2011.01.002. Epub 2011 Jan 22.
Electrostatic interaction is an important secondary force affecting the structure, stability, and function of lipid vesicles (liposomes). For this study, a negatively charged lipid with carboxylic acid was mixed with phospholipid to produce anionic vesicles. The electrostatics of the carboxylated anionic vesicle (ca. 200 nm diameter) was determined and correlated with entrapment capacity of the vesicles. Correlative analysis revealed the zeta potential of the vesicles as a factor quantitatively affecting the entrapment capacity for a water-soluble marker, in which the entrapment capacity reached its maximum level in less than -30 mV of zeta potential. Transmission electron microscopy (TEM) revealed that the vesicles with high entrapment capacity are composed of a unilamellar membrane. This finding is expected to be useful for efficient encapsulation of water-soluble pharmaceuticals within vesicles.
静电相互作用是影响脂质体（脂质囊泡）结构、稳定性和功能的重要次级作用力。在这项研究中，将带负电荷的羧酸脂质与磷脂混合以产生阴离子囊泡。测定了带负电荷的羧基阴离子囊泡（直径约 200nm）的静电力，并将其与囊泡的包封能力相关联。相关分析显示，囊泡的 ζ 电位是定量影响水溶性标记物包封能力的因素，其中在 ζ 电位小于-30mV 时，包封能力达到最大值。透射电子显微镜（TEM）显示，具有高包封能力的囊泡由单层膜组成。这一发现有望用于高效包封水溶性药物于囊泡内。
