Soybean Research Institute, National Center for Soybean Improvement, National Key Laboratory for Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China.
Virus Res. 2011 Apr;157(1):13-8. doi: 10.1016/j.virusres.2011.01.011. Epub 2011 Jan 22.
Two soybean viral isolates 4469-4 and Sc6 from plants in China were characterized by serological assays, pathogenicity tests, full-genome sequencing and sequence analyses. Both isolates are determined to be Soybean mosaic virus (SMV) isolates but 4469-4 is different from other known SMVs by inducing symptoms on common bean. 4469-4 has an RNA genome of 9994 nucleic acids (nt) encoding 3202 amino acids (aa), which is approximately 400 nt longer than that of Sc6 and other SMV strains. Comparison with SMV and SMV-related potyviruses suggests that 4469-4 shares high nt and aa sequence identify (>92%) with other SMV strains. However, significant diversity between 4469-4 and other SMV strains was observed in the 5' genomic region. In contrast, this region is highly similar to the corresponding region of Bean common mosaic virus (BCMV). Recombination analyses conclude that there is a recombination site near the nt 900 of 4469-4. Taken together these data suggest that 4469-4 may result from recombination between SMV and BCMV or a BCMV-like virus in the N-terminus of the genome.
两段大豆病毒分离物 4469-4 和 Sc6 来自中国的植物，通过血清学检测、致病性测试、全基因组测序和序列分析进行了特征描述。这两个分离物均被确定为大豆花叶病毒（SMV）分离物，但 4469-4 通过在普通菜豆上诱导症状与其他已知的 SMV 不同。4469-4 的 RNA 基因组为 9994 个核苷酸（nt），编码 3202 个氨基酸（aa），比 Sc6 和其他 SMV 株系长约 400nt。与 SMV 和 SMV 相关的马铃薯 Y 病毒属病毒的比较表明，4469-4 与其他 SMV 株系的 nt 和 aa 序列具有高度相似性（>92%）。然而，4469-4 与其他 SMV 株系在 5'基因组区域存在显著差异。相比之下，该区域与 Bean common mosaic virus（BCMV）的相应区域高度相似。重组分析得出结论，4469-4 在 nt900 附近存在一个重组位点。综合这些数据表明，4469-4 可能是由 SMV 和 BCMV 或基因组 N 端的 BCMV 样病毒之间的重组产生的。
