Zhao Tiantian, Zhang Yuhang, Wang Fengmin, Zhang Bo, Chen Qiang, Liu Luping, Yan Long, Yang Yue, Meng Qingmin, Huang Jinan, Zhang Mengchen, Lin Jing, Qin Jun
Hebei Laboratory of Crop Genetics and Breeding, National Soybean Improvement Center Shijiazhuang Sub-Center, Huang-Huai-Hai Key Laboratory of Biology and Genetic Improvement of Soybean, Ministry of Agriculture and Rural Affairs, Institute of Cereal and Oil Crops, Hebei Academy of Agricultural and Forestry Sciences, Shijiazhuang, 050035 China.
Guangdong Key Laboratory of Plant Adaptation and Molecular Design, Guangzhou Key Laboratory of Crop Gene Editing, Innovative Center of Molecular Genetics and Evolution, School of Life Sciences, Guangzhou University, Guangzhou Higher Education Mega Center, 230 Waihuanxi Road, 510006 Guangzhou, China.
Mol Breed. 2023 Jan 16;43(2):7. doi: 10.1007/s11032-022-01351-3. eCollection 2023 Feb.
Necrosis caused by soybean mosaic virus (SMV) has not been specifically distinguished from susceptible symptoms. The molecular mechanism for the occurrence of necrosis is largely overlooked in soybean genetic research. Field evaluation reveals that SMV disease seriously influences soybean production as indicated by decreasing 22.4% ~ 77.0% and 8.8% ~ 17.0% of yield and quality production, respectively. To expand molecular mechanism behind necrotic reactions, transcriptomic data obtained from the asymptomatic, mosaic, and necrotic pools were assessed. Compared between asymptomatic and mosaic plants, 1689 and 1752 up- and down-regulated differentially expressed genes (DEGs) were specifically found in necrotic plants. Interestingly, the top five enriched pathways with up-regulated DEGs were highly related to the process of the stress response, whereas the top three enriched pathways with down-regulated DEGs were highly related to the process of photosynthesis, demonstrating that defense systems are extensively activated, while the photosynthesis systems were severely destroyed. Further, results of the phylogenetic tree based on gene expression pattern and an amino acid sequence and validation experiments discovered three PR1 genes, , , and , which were especially expressed in necrotic leaves. Meanwhile, exogenous salicylic acid (SA) but not methyl jasmonate (MeJA) could induce the three PR1 gene expressions on healthy leaves. Contrastingly, exogenous SA obviously decreased the expression level of , , and concentration of SMV, but increased expression in necrotic leaves. These results showed that is associated with the development of SMV-induced necrotic symptoms in soybean. , , and is up-regulated in necrotic leaves at the transcriptional levels, which will greatly facilitate a better understanding of the mechanism behind necrosis caused by SMV disease.
The online version contains supplementary material available at 10.1007/s11032-022-01351-3.
由大豆花叶病毒（SMV）引起的坏死尚未与易感症状明确区分。在大豆遗传研究中，坏死发生的分子机制在很大程度上被忽视。田间评估表明，SMV病害严重影响大豆产量，产量和品质产量分别下降22.4%至77.0%和8.8%至17.0%。为了拓展坏死反应背后的分子机制，对从无症状、花叶和坏死库中获得的转录组数据进行了评估。在无症状和花叶植株之间进行比较，在坏死植株中分别特异性地发现了1689个上调和1752个下调的差异表达基因（DEG）。有趣的是，上调DEG的前五个富集通路与应激反应过程高度相关，而下调DEG的前三个富集通路与光合作用过程高度相关，这表明防御系统被广泛激活，而光合作用系统被严重破坏。此外，基于基因表达模式和氨基酸序列的系统发育树结果以及验证实验发现了三个PR1基因，即 、 和 ，它们在坏死叶片中特异性表达。同时，外源水杨酸（SA）而非茉莉酸甲酯（MeJA）可诱导这三个PR1基因在健康叶片上表达。相反，外源SA明显降低了 、 和 的表达水平以及SMV的浓度，但增加了坏死叶片中 的表达。这些结果表明， 与大豆中SMV诱导的坏死症状的发展有关。 、 和 在坏死叶片中的转录水平上调，这将极大地有助于更好地理解SMV病害引起坏死的机制。
在线版本包含可在10.1007/s11032-022-01351-3获取的补充材料。
