Department of Chemistry and Applied Biosciences, ETH Zürich, Zurich, Switzerland.
J Proteomics. 2011 Apr 1;74(4):539-46. doi: 10.1016/j.jprot.2011.01.010. Epub 2011 Jan 22.
Assessing protein changes in the cerebral vasculature of brain disorders may increase our understanding of disease pathogenesis and facilitate diagnostic and therapeutic intervention. By combining perfusion of mice with a charged reactive biotin derivative and subsequent quantification of the biotinylated proteins, the proteome accessible from the vasculature in an APPPS1 transgenic mouse model of cerebral β-amyloidosis was identified and compared to that in non-transgenic control mice. Our results provide proof-of-concept of this technology for the identification of new targets for antibody-based therapy or pharmacodelivery, and for neuroimaging in neurodegenerative diseases.
评估脑部疾病中脑血管的蛋白质变化可能会增进我们对疾病发病机制的了解，并有助于诊断和治疗干预。通过将小鼠的灌注与带电荷的反应性生物素衍生物结合，并随后对生物素化蛋白质进行定量，我们鉴定了 APPPS1 转基因小鼠模型中脑β-淀粉样蛋白相关脑血管中可及的蛋白质组，并与非转基因对照小鼠进行了比较。我们的结果为该技术在鉴定用于基于抗体的治疗或药物递送的新靶标以及在神经退行性疾病中的神经影像学方面提供了概念验证。
