用肝毒素甲酸烯丙酯处理的大鼠心脏和血浆中对氨基脲敏感的胺氧化酶的比较研究。

Comparative studies on semicarbazide-sensitive amine oxidase in heart and plasma of rats treated with hepatotoxin allyl formate.

Obata T, Yamanaka Y

Department of Pharmacology, Medical College of Oita, Japan.

Int J Biochem. 1990;22(8):837-9. doi: 10.1016/0020-711x(90)90286-c.

Abstract

After allyl formate (AF) was administered to the rats, the existence of semicarbazide-sensitive amine oxidase (SSAO) in rat identified. 2. When the heart homogenate and plasma of AF-administered rat were pretreated with 10(-3) M clorgyline and deprenyl, the Km value for benzylamine of rat heart was same as the value of plasma. 3. The existence of SSAO in plasma of AF-administered rats were identified by IEF-gel electrophoresis. The pI values of SSAO in heart and plasma were a single peak of 5.0. 4. SSAO released from the rat heart in response to AF, although the other origins of this enzyme are unknown.

摘要

将甲酸烯丙酯（AF）给予大鼠后，在大鼠体内鉴定出了氨基脲敏感胺氧化酶（SSAO）的存在。2. 当用10⁻³ M的氯吉兰和司来吉兰预处理给予AF的大鼠的心脏匀浆和血浆时，大鼠心脏中苄胺的Km值与血浆中的值相同。3. 通过IEF-凝胶电泳鉴定了给予AF的大鼠血浆中SSAO的存在。心脏和血浆中SSAO的pI值为单一峰值5.0。4. 尽管该酶的其他来源尚不清楚，但AF会使大鼠心脏释放出SSAO。