Department of Microbiology, School of Medicine, Iwate Medical University, Morioka, Iwate, Japan.
J Biosci Bioeng. 2011 May;111(5):600-4. doi: 10.1016/j.jbiosc.2010.12.018. Epub 2011 Jan 23.
A microcarrier is used for the three-dimensional (3D) culture of adhesion-dependent mammalian cells. We developed a novel microcarrier by binding ProNectin F, an artificial cell adhesive protein synthesized by genetically engineered Escherichia coli to a polyacrylic superabsorbent polymer. The microcarrier is characterized by containing no animal-derived components. The serum-free culture of Vero cells for vaccine production using the microcarrier increased the number of Vero cells by approximately 30% compared with the existing dextran beads coated with porcine Type I collagen, which resulted in approximately a 30% to 40% increase in the infectivity titer of the Sabin 2 strain of poliovirus. These results suggested that the developed microcarrier should be unprecedented in permitting high-yield vaccine production by means of a serum-free culture.
微载体用于依附性哺乳动物细胞的三维（3D）培养。我们通过将人工细胞黏附蛋白 ProNectin F 与聚丙烯酸超吸收聚合物结合，开发出一种新型微载体。该微载体的特点是不含动物源性成分。使用该微载体进行无血清培养生产疫苗时，与现有的用猪 I 型胶原包被的葡聚糖珠相比，Vero 细胞的数量增加了约 30%，导致脊髓灰质炎病毒 Sabin 2 株的感染力滴度增加了约 30%至 40%。这些结果表明，通过无血清培养，开发的微载体应该能够前所未有地实现高产疫苗生产。
