Aquatic Animal Health Research Group, Faculty of Biosciences and Aquaculture, University of Nordland, Bodø, Norway.
Comp Biochem Physiol Part D Genomics Proteomics. 2011 Jun;6(2):158-62. doi: 10.1016/j.cbd.2011.01.001. Epub 2011 Jan 7.
The present study describes the transcriptional profiles of selected immune and stress genes with putative important roles in the cutaneous immune defense of Atlantic cod (Gadus morhua). In addition it shows differential expression of many genes at the dorsal and ventral sides of fish, in general having the highest expression at the latter side. Genes related to antibacterial activity, antiviral response, cytokine production, glucose transport, stress response and anti-apoptotic activity were monitored and bactericidal/permeability-increasing protein/lipopolysaccharide-binding protein (BPI-LBP), g-type lysozyme, transferrin, metallothionein, fortilin, interferon regulatory factor-1 (IRF-1), a CC chemokine isoform, interleukin-8 (IL-8), glucose transport (GLUT)-1, -3 and -4, Cu,Zn-superoxide dismutase (Cu,Zn-SOD), catalase and hsp 70 showed significantly higher expression at the ventral side. Further g-type lysozyme, metallothionein, fortilin, IRF-1, interferon γ, interleukin-1β (IL-1β), GLUT-3 and -4, catalase and anti apoptotic gene Bcl-X1 were highly expressed in adult cod skin. Therefore fish skin can be considered an immunological active site, especially at the ventral side of Atlantic cod.
本研究描述了具有潜在重要作用的选择免疫和应激基因的转录谱，这些基因在大西洋鳕鱼（Gadus morhua）的皮肤免疫防御中。此外，它还显示了鱼类背部和腹部许多基因的差异表达，通常后者的表达最高。监测了与抗菌活性、抗病毒反应、细胞因子产生、葡萄糖转运、应激反应和抗凋亡活性相关的基因，杀菌/通透性增加蛋白/脂多糖结合蛋白（BPI-LBP）、g 型溶菌酶、转铁蛋白、金属硫蛋白、for tilin、干扰素调节因子-1（IRF-1）、CC 趋化因子同工型、白细胞介素-8（IL-8）、葡萄糖转运（GLUT）-1、-3 和 -4、Cu、Zn-超氧化物歧化酶（Cu、Zn-SOD）、过氧化氢酶和 hsp 70 在腹部的表达明显更高。此外，g 型溶菌酶、金属硫蛋白、for tilin、IRF-1、干扰素γ、白细胞介素-1β（IL-1β）、GLUT-3 和 -4、过氧化氢酶和抗凋亡基因 Bcl-X1 在成年鳕鱼皮肤中高度表达。因此，鱼类皮肤可以被认为是一个免疫活性部位，特别是在大西洋鳕鱼的腹部。
