Nalesnik Milada, Nikolić Jasminka Mehanović, Jandrić Slavica
School of Medicine of the University of Banjaluka, Banjaluka, Bosnia and Herzegovina.
Med Glas (Zenica). 2011 Feb;8(1):163-8.
To determine of catalytic activities of adenosine deaminase (ADA) and values of C-reactive protein (CRP) concentration in serums of patients with rheumatoid arthritis (RA), who were and were not treated with Methotrexate (MTX), and identifying the possibilities of using these biochemical parameters in diagnosing and monitoring of treatment effects in RA.
The study involved 120 subjects (60 healthy ones, who are in accordance with examined groups concerning age and sex, 30 suffering from RA who were not treated with MTX and 30 suffering from RA who were treated by MTX). Catalytic activities of ADA in serum were determined by spectrophotometric method using adenosine as a substrate. CRP concentrations in serum were determined immunoturbidimetrically.
A statistically significant correlation between values of ADA catalytic activities and values of CRP concentrations (r = 0.55, p < 0.01) in serums of subjects with RA without MTX treatment. At subjects with RA treated by MTX, correlation between values of ADA catalytic activities and values of CRP concentrations in serums was not statistically significant (r = 0.33, p > 0.01).
Study results have shown that ADA catalytic activity in serum can be a useful biochemical marker of inflammatory process in RA.
测定类风湿关节炎（RA）患者血清中腺苷脱氨酶（ADA）的催化活性及C反应蛋白（CRP）浓度值，这些患者接受或未接受甲氨蝶呤（MTX）治疗，并确定利用这些生化参数诊断RA及监测治疗效果的可能性。
该研究纳入120名受试者（60名健康者，其年龄和性别与受试组相匹配，30名未接受MTX治疗的RA患者以及30名接受MTX治疗的RA患者）。以腺苷为底物，采用分光光度法测定血清中ADA的催化活性。采用免疫比浊法测定血清中CRP的浓度。
未接受MTX治疗的RA患者血清中，ADA催化活性值与CRP浓度值之间存在统计学显著相关性（r = 0.55，p < 0.01）。在接受MTX治疗的RA患者中，血清中ADA催化活性值与CRP浓度值之间的相关性无统计学意义（r = 0.33，p > 0.01）。
研究结果表明，血清中ADA催化活性可能是RA炎症过程的有用生化标志物。
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